Intitulé de l'offre : STORM (Direct STochastic Optical Reconstruction Microscopy) microbiology/microscopy researcher (M/W) (H/F)
Référence : UMR5075-VALLAN-017
Nombre de Postes : 1
Lieu de travail : GRENOBLE
Date de publication : mardi 21 novembre 2023
Type de contrat : CDD Scientifique
Durée du contrat : 12 mois
Date d'embauche prévue : 1 mars 2024
Quotité de travail : Temps complet
Rémunération : Between 2 905.76 € et 4 081.77 € (according to experience)
Niveau d'études souhaité : Niveau 8 - (Doctorat)
Expérience souhaitée : 1 à 4 années
Section(s) CN : Molecular and structural biology, biochemistry
The main activity of the person recruited will be to carry out experiments linked to the ANR "NANOWALL" project. The post-doctoral project aims to elucidate the dynamics of the synthesis and maturation processes for the two major components of the cell wall of Gram-positive bacteria, peptidoglycan (PG) and teichoic acids (TA), and to discover the key interactions that connect the assembly of these two components. It is based on a combination of click chemistry methods, one- and two-colour super-resolved fluorescence microscopy and biochemistry, applied to wild-type and mutant strains of the human pathogen Streptococcus pneumoniae.
1. Carrying out research work, from setting up the experiment to writing up the results:
- monitor the project's literature
- cultivate S. pneumoniae cells in which the PG and TA will be labelled with clickable compounds
- collect and analyze conventional fluorescence microscopy data (low resolution)
- collect, process and analyze dSTORM (direct STochastic Optical Reconstruction Microscopy) data
- prepare extracts of labelled bacterial cell wall (PG and TA) and analyze them by SDS-PAGE
- prepare results for monthly reports and oral presentations in English
- make suggestions for the development and improvement of experiments
- write research protocols and articles in English for publication.
2. Participate in the life of the laboratory :
- taking part in the life of the research group (contributing to logistics, monitoring stocks of reagents, tidying up, etc.)
- help supervising trainees
- taking part in the Institute's scientific events
1) Fundamental knowledge
- PhD in structural biology/cell biology with good knowledge of microbiology
- In-depth knowledge of fundamental microbiology, molecular mechanisms of bacterial division, cell wall composition, etc.
- General knowledge of click chemistry processes
- General knowledge of fluorescence microscopy methods
- English language: B2 preferred (according to the Common European Framework of Reference for Languages)
2) Technical skills
- Excellent technical skills
- Experience of fluorescence microscopy techniques
- Knowledge of data processing and IT applied to image analysis
- Knowledge of single molecule data processing (dSTORM)
- Mastery of statistical analysis
- Knowledge of and compliance with health and safety regulations in force in laboratories, particularly in P2 laboratories
3) Behavioral and interpersonal skills
- Ability to work independently
- Pro-activity in research, scientific curiosity
- Open-mindedness and ability to listen
- Good interpersonal skills and ability to integrate and adapt to the team
- Organizational skills and rigor
- Good communication skills (written and oral) and ability to report on your work
Contexte de travail
Located on the Grenoble Science Park (ESRF/ILL/EMBL/IBS), the Institute of Structural Biology (IBS) is a national and international player in the field of integrated structural biology. The IBS is a research center, a technical platform, a host site and a scientific training center. Its mission is to develop research in structural biology, a field of research that is crucial to the understanding of fundamental biological mechanisms. It is supported by 12 state-of-the-art platforms.
As a joint research unit (CEA-CNRS-UGA), the IBS is composed of 19 research groups, each of which proposes a multi-disciplinary approach, at the frontiers of biology, physics and chemistry, in coherence with 3 research axes. Nearly 300 people work here (researchers, doctoral students, engineers, technicians and students) in a multicultural and international environment.
The person recruited will join the Pneumococcus group, which is working on the molecular mechanisms of bacterial morphogenesis during growth and division in S. pneumoniae and during sporulation in B. subtilis (https://www.ibs.fr/research/research-groups/pneumococcus-group-c-morlot/).
Contraintes et risques
The research will be carried out in either French or English, however it is expected that the non-French speaking candidate will strive to learn the basics of French in order to facilitate communication and integration into the laboratory.