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Portal > Offres > Offre UMR5075-JOSRAM-018 - Chercheur en biologie structurale de la chromatine (H/F)

Researcher in the structural biology of chromatin (M/F)

This offer is available in the following languages:
Français - Anglais

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General information

Reference : UMR5075-JOSRAM-018
Workplace : GRENOBLE
Date of publication : Monday, July 29, 2019
Type of Contract : FTC Scientist
Contract Period : 24 months
Expected date of employment : 1 October 2019
Proportion of work : Full time
Remuneration : 2617 euros gross per month
Desired level of education : PhD
Experience required : 1 to 4 years

Missions

The researcher will use a biophysical and structural approach to study post-meiotic genome reorganization, which involves the eviction of histones from nucleosomes and their replacement by transition proteins and protamines. The study will focus on the transitional states that appear in post-meiotic haploid cells when nucleosomes are transformed into nucleoprotamines, as described in Mol Cell 2017 66:89-101, PMID 28366643. The work will include purifying endogenous macromolecular complexes from mammalian cells as well as reconstituting complexes using recombinantly expressed proteins, and subsequently using this material for biophysical analyses and for low- and high-resolution 3D structure determination.

Activities

Activities include:
- purification of protein/nucleic acid complexes from endogenous sources;
-expression and purification of recombinant proteins and reconstitution of macromolecular complexes;
- biophysical assays; crystallization trials and crystal structure determination;
- negative-stain and cryo-electron microscopy.

Skills

- experience in biochemistry and structural biology
- ability to work independently and collaboratively on an interdisciplinary project
- ability to analyze, organize and publish research data
- experience in the chromatin/epigenetics field is an asset

Work Context

The IBS is a French academic research unit, affiliated with the University Grenoble-Alpes, the CNRS and the CEA. It currently employs 280 people, including many students and post-doctoral researchers of diverse nationality. The IBS possesses high-performance internationally competitive facilities in molecular and cellular biology, biochemistry, biophysics and structural biology. The Institute is a founding member of the Partnership for Structural Biology together with three European institutes (the European Synchrotron Radiation Facility, the Institut Laue-Langevin for neutron studies and the Grenoble Outstation of the EMBL), all located on the same campus and providing a strong international environment. Grenoble is a pleasant city situated in the French Alps and is one of the most renowned centres in France for structural biology research.

Constraints and risks

The researcher will work in the "Viral infection and cancer" group at the Institute for Structural Biology (IBS) in close collaboration with the "Epigenetics and cell signalling" team of the Institute for Advanced Biosciences.

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