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Portail > Offres > Offre UMR5075-JERGAU-010 - Chercheur postdoctoral en caractérisation structurale de la machinerie de biosynthèse de l'héparane sulfate (H/F)

Post-doctoral researcher in Structural characterization of the heparan sulfate biosynthesis machinery (H/F)

This offer is available in the following languages:
Français - Anglais

Date Limite Candidature : mercredi 30 juin 2021

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General information

Reference : UMR5075-JERGAU-010
Workplace : GRENOBLE
Date of publication : Wednesday, June 9, 2021
Type of Contract : FTC Scientist
Contract Period : 24 months
Expected date of employment : 1 September 2021
Proportion of work : Full time
Remuneration : According to experience and from € 2648 gross
Desired level of education : PhD
Experience required : 1 to 4 years

Missions

An ATIP-Avenir funded postdoc position is available in our newly established team at the IBS in Grenoble, France.

The Wild team has a keen interest in understanding the mechanisms of enzymes involved in heparan sulfate biosynthesis on a molecular level. Heparan sulfates are long and highly complex polysaccharide chains found on the cell surface and their biosynthesis involves more than a dozen of Golgi-localized membrane-anchored enzymes. Our team combines in vitro biochemical assays with structural biology approaches, in particular single-particle cryo-EM, to study the molecular mechanisms of the heparan sulfate biosynthesis enzymes and their assembly into macromolecular complexes.

The IBS (Institut de Biologie Structurale) is located together with the EMBL Grenoble and the ESRF synchroton facility at the EPN campus and has excellent infrastructure for pursuing challenging structural biology projects and a great scientific community. The team has regular access to a 200kV Glacios and 300kV Titan Krios TEM for grid screening and data collection.

We are looking for a highly motivated and team-oriented candidate with a PhD in structural biology or protein biochemistry. A profound knowledge in expressing and purifying proteins is expected, first experiences in cryo-EM data collection, processing and model building would be an advantage. In addition, the candidate is expected to have excellent written and oral communication skills in English and at least one first-author publications.

Activities

1) Protein purification and characterization
- recombinant protein expression in mammalian cell systems
- purification of soluble and membrane-embedded proteins
- enzyme activity assays to check functionality of purified proteins
- biophysical methods to study protein-protein & protein-ligand interactions

2) Structure determination
- sample preparation for cryo-EM data collection
- EM grid screening and data collection on the in-house 200kV Glacios TEM
- data processing, interpretation and model building

3) Manuscript writing and oral presentation
- summarizing results for publication in peer-reviewed journals
- presentation of results on national or international conferences

Skills

1) Scientific knowledge
- Strong understanding of enzymatic reaction mechanisms and protein structure-function relationships
- Familiarity with different types of glycosylation found in cells

2) Technical know-how
- Cryo-EM grid preparation, data collection and processing
- Protein purification using FPLC, equipped with various types of chromatography columns
- Working with mammalian cells under sterile conditions
- Sample characterization by Western Blot, nanoDSF, SPR, ITC, MST, ...

3) Behavioural and relational skills
- Being comfortable to work in a small, newly established team
- Excellent English skills (B2 level required), English is the daily working language of the team
- Work efficiently together with other lab members and collaborators on a project
- Ability to establish new work-flows
- Creative and solution-oriented trouble shooting
- Technical rigour and good organisational skills

Work Context

The IBS (300 people) is a joint CEA-CNRS-UGA research unit located in the scientific environment of the Grenoble presqu'ile, which houses CEA and CNRS units, as well as the European laboratories of the ILL, ESRF and EMBL, creating a dynamic local synergy through strong partnerships (Partnership for Structural Biology - PSB).

The SAGAG group (Structure and Activity of Glycosaminoglycans group) is composed of three teams and has a long-standing, internationally recognized expertise in characterizing heparan sulfate structures and their interaction with proteins. The newly established Wild team focusses on the functional and structural characterization of enzymes involved in heparan sulfate biosynthesis and the Postdoc candidate will be expected to work closely together with other group members to advance and complete ongoing projects.

The team has direct access to several platforms and facilities, including a well-equipped electron microscopy facility, protein crystallization, mass-spectrometry and several instruments for the biophysical characterization of proteins.

Constraints and risks

none

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