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Portail > Offres > Offre UMR5075-JERGAU-008 - Chercheur postdoctoral en microscopie corrélative FIB/SEM - Cryo-TEM appliquée a l'étude des photosymbioses dans le plancton marin (H/F)

Post-doctoral researcher in correlative microscopy FIB/SEM - Cryo-TEM applied to the study of photosymbiosis in marine pklankton (H/F)

This offer is available in the following languages:
Français - Anglais

Date Limite Candidature : mercredi 19 mai 2021

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General information

Reference : UMR5075-JERGAU-008
Workplace : GRENOBLE
Date of publication : Wednesday, April 28, 2021
Type of Contract : FTC Scientist
Contract Period : 18 months
Expected date of employment : 1 July 2021
Proportion of work : Full time
Remuneration : According to experience and from € 2648 gross
Desired level of education : PhD
Experience required : 1 to 4 years

Missions

The recruited researcher will play a central role in a research project with a very strong partnership entitled SymbioChip. The main objective of the project is to develop high-resolution 3D imaging in cryo mode on photosymbiosis in marine plankton. More specifically, the candidate will have to develop a sample preparation protocol with correlative microscopy, including cryofixation and tomographic imaging (FIB-SEM and cryo-TEM). These protocols will be tested on different samples and the results evaluated by the above-mentioned imaging methods. Once developed, these protocols will be applied to various marine microalgae involved in photosymbiosis.

Activities

- Implementation of a workflow in cryo mode for 3D electron microscopy
- Cryofixation and cryo fluorescence microscopy
- Cryo transmission electron microscopy
- Sampling of plankton at sea with partner laboratories
- Communication through international meetings and workshops.

Skills

1) Scientific knowledge
- PhD in Life Sciences
- Knowledge on plankton and symbioses

2) Operational skills
- Knowledge and expertise in sample preparation for electron microscopy (FIB-SEM, TEM) and fluorescence microscopy in cryo mode: fixation, sectioning.
- Ability to sample plankton at sea and to isolate cells under the microscope

3) Interpersonal skills
- Autonomy
- Ability to interact and maintain productive relationships with different audiences, internally (research group) + externally (project partners + the rest of the scientific community)
- Ability to provide expertise in a multidisciplinary context, to have a good scientific culture
- Written and oral scientific English.

Work Context

Located on the EPN site (ESRF/ILL/EMBL/IBS) on the Grenoble Scientific Peninsula, the Institute of Structural Biology (IBS) is a joint research unit of 280 people from CNRS, the UGA and the CEA.
The IBS is a research center, a technical platform, a host site and a scientific training center. Its mission is to develop research in structural biology, a field of research that is crucial to the understanding of fundamental biological mechanisms. The research activity of IBS is structured in 3 axes and 19 research groups. These teams are supported by a state-of-the-art research infrastructure that includes 12 platforms.
This project is a collaborative project between IBS (Electron Microscopy and Methods group, MEM) and the Photosymbiosis team (photosymbiosis.com) of the Celland Plant Physiology laboratory (LPCV: UMR 5168 - CNRS -CEA - Univ. Grenoble Alpes - INRA) within the IRIG institute of the CEA in Grenoble.

The candidate will work at IBS in the MEM group in strong interaction with the Photosymbiosis team. The MEM group seeks to develop its expertise in 3D cell imaging by electron microscopy and the Photosymbiosis team will provide the samples and expertise on symbiosis. The development and application of sample preparation methods is crucial to understand the functioning of photosynthesis in unicellular photosymbiosis.

He/she will be able to rely on the expertise of the IBS cell electron microscopy platform which is attached to the MEM group. In addition to the interaction between IBS and PCV, the candidate will also interact with the INAC platform (IRIG) for FIB-SEM studies.

This recruitment is part of an international project led by the Roscoff Biological Station SymbioChip and funded by The Gordon and Betty Moore Foundation (more info here). The candidate will interact regularly with the project partners during meetings (Station Biologique de Roscoff (CNRS-Sorbonne Univ), Bigelow Laboratory for Ocean Sciences, and Jena Friedrich-Schiller University) and will also participate in field sampling (Villefranche sur Mer) which will provide the material to be studied.

Usefull link:

Fondation Moore:
https://www.moore.org/initiative-strategy-detail?initiativeId=symbiosis-in-aquatic-systems-initiative

IBS electron microscopy team
https://www.ibs.fr/research/research-groups/methods-and-electron-microscopy-group/schoehn-team/

IBS EM plaform
https://www.ibs.fr/research/research-groups/methods-and-electron-microscopy-group/electron-microscopy-platform/article/presentation-of-the-tem-platform-3601

Photosymbiosis team
https://photosymbiosis.com/

Roscoff Marine Station
http://www.sb-roscoff.fr/

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