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Reference : UMR5075-JERGAU-005
Workplace : GRENOBLE
Date of publication : Friday, February 05, 2021
Type of Contract : FTC Technical / Administrative
Contract Period : 18 months
Expected date of employment : 1 April 2021
Proportion of work : Full time
Remuneration : According to experience and from 2437 € gross
Desired level of education : PhD
Experience required : Indifferent
The research project proposed aims at characterizing the mechanisms of heparan sulfate (HS) biosynthesis. These molecules, present on the cell surfaces, control many biological processes such as cell proliferation, adhesion and migration, inflammation and immune response, development or host/pathogen interactions.
The candidate will join a team (The SAGAG group at IBS) of about ten people, at the forefront of HS research, with a first-rate technological environment. In this context, his/her role, in close interaction with the laboratory members, will be to study the functional and structural aspects of some of the enzymes involved in HS biosynthesis in order to better understand how these molecules carry out their numerous biological activities.
The candidate will :
- Express and purify recombinant enzymes
- Prepare oligosaccharide enzyme substrates
- Implement biochemical assays to study enzymes biological activities and interactions with protein partners.
- Develop mutagenesis and structural biology approaches to identify the active and to characterize enzyme-substrate and enzyme-partner complexes.
The candidate, holder of a recently obtained thesis should have :
- A good general knowledge of biochemistry and enzymology
- A good expertise in expression (in eukaryotic cells), purification (chromatography) and characterization (electrophoresis, spectrometry) of proteins.
- An expertise in analytical methods (HPLC) for monitoring enzymatic reactions.
- An expertise in crystallogenesis and biochemistry of glycans will be a plus
The candidate will also have to show an ability to work independently, but in close collaboration with the other group members
The IBS is a joint research unit under the supervision of the University of Grenoble-Alpes, the CNRS and the CEA. More than 300 people work at the IBS, including 180 permanent employees, numerous students and post-doctoral fellows from different countries.
The IBS has efficient and internationally competitive facilities in the fields of molecular and cellular biology, biochemistry, biophysics and structural biology. The Institute is a founding member of the Partnership for Structural Biology (PSB) with prestigious pan-European institutes: the European Synchrotron Light Source (ESRF), the European Neutron Scattering Centre (ILL) and the EMBL station in Grenoble, all located nearby and offering a strong international environment.
Within the IBS, the recruited person will join the "Structure and Activity of Glycosaminoglycans" Group (SAGAG) headed by Hugues LORTAT-JACOB (https://www.ibs.fr/recherche/groupes-de-recherche/groupe-structure-et-activite-des-glycosaminoglycanes-h-lortat-jacob/).
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