Faites connaître cette offre !
Reference : UMR5075-JEASIM-001
Workplace : GRENOBLE
Date of publication : Wednesday, October 02, 2019
Type of Contract : FTC Scientist
Contract Period : 18 months
Expected date of employment : 1 January 2020
Proportion of work : Full time
Remuneration : 3000 - 3729 euros
Desired level of education : PhD
Experience required : 1 to 4 years
Innovative spectroscopic approaches based on MAS-DNP will be conducted to provide new tools for the investigation of protein interaction with the bacterial cell wall. This project aims at developing the available MAS-DNP technique in order to provide best spectral sensitivity and resolution for the investigation of the bacterial cell wall itself in extracted and entire cells, as well as possible interactions with proteins involved in L,D-transpeptidation. These developments will make use of state-of-the-art DNP methods and polarizing agents developed or under development in the lab, which hosts two 400 MHz MAS-DNP spectrometers.
-The task will consist in the study of the bacterial cell wall of Mycobacteria and its interaction with L,D-transpeptidase by MAS-DNP. It will include different experimental aspects: optimizing the DNP sample preparation of PG and entire bacterial cells, testing the behavior of different paramagnetic species and their impact on the resonances from the cell wall and interaction partners, use of advanced DNP techniques for best sensitivity, resolution and selectivity for the PG-protein binding site. Analysis of the multidimensional spectra and modeling of the binding site after extraction of structural constraints will be performed with specialized software used for biomolecular NMR. Growing of cells and preparation of extracted PG may be required.
Applicants are expected to have a doctoral experience in liquid-state and/or solid-state biomolecular NMR spectroscopy. Knowledge about MAS-DNP will be considered as a plus.
This research will fit in a collaborative effort led by two internationally recognized NMR research groups in Grenoble, which covers a wide spectrum of competences going from high-field liquid-state NMR to advanced MAS-DNP : the team directed by Dr Jean-Pierre Simorre in the Biomolecular NMR Spectroscopy group at Institut de Biologie Structurale, and the DNP team of the Interdisciplinary Research Institute in Grenoble, directed by Gaël De Paëpe. The DNP team hosts two 400-MHz MAS-DNP spectrometers (one equipped with a helium-recirculated cooling system) and is a pioneer in instrumentation and methods developments for MAS-DNP.
Grenoble is one of the major cities in Europe for research with a large international scientific community. In addition, Grenoble has a large international student population, is a very pleasant city to live in, and is known as the “Capital of the Alps” with easy access to great skiing and hiking. It is also only 2 hours' drive to the Mediterranean Sea, Italy, or Switzerland. Grenoble, Lyon, and Geneva airports are nearby and permit straightforward international travel.
Constraints and risks
Experiments recorded on high magnetic field spectrometers
We talk about it on Twitter!