Missions

Structural characterization of enzymes involved in chondroitin sulfate biosynthesis using cryo-electron microcopy.

Activités

- expression of recombinant proteins in human HEK293F cell lines
- purification and biophysical charcterization of enzymes
- structure determination by single-particle cryo-EM
- training and supervision of PhD students

Compétences

- detailed knowlegde on glycosaminoglycan biosynthesis and function
- experience in protein purification from mammalian expression systems
- autonomous in cryo-EM grids preparation and screening
- solid expertise in cryo-EM data analysis
- English (written/oral) level C1

Contexte de travail

Located on the Grenoble Science Park (ESRF/ILL/EMBL/IBS), the Institute of Structural Biology (IBS) is a national and international player in the field of integrated structural biology. The IBS is a research center, a technical platform, a host site and a scientific training center. Its mission is to develop research in structural biology, a field of research that is crucial to the understanding of fundamental biological mechanisms. It is supported by 12 state-of-the-art platforms.
As a joint research unit (CEA-CNRS-UGA), the IBS is composed of 20 research groups, each of which proposes a multi-disciplinary approach, at the frontiers of biology, physics and chemistry, in coherence with 3 research axes. Nearly 320 people work there as researchers, PhD students, engineers and technicians in a multicultural and international environment.
The person recruited will join the team of Rebekka Wild in the 'Structure and Activity of Glycosaminoglycans' (SAGAG) group. The team is interested in understanding the molecular mechanisms of enzymes involved in the biosynthesis of glycosaminoglycans (GAGs). GAGs are long chains of highly complex polysaccharides found on the cell surface, and their biosynthesis involves more than a dozen membrane-bound enzymes localized in the Golgi apparatus. Our team combines in vitro biochemical analyses with structural biology approaches, in particular single-particle cryo-EM, to study the molecular mechanisms of GAG biosynthesis enzymes and their assembly into macromolecular complexes.

Contraintes et risques

Work in laboratory with safety level 2.
The research will be carried out in either French or English, however it is expected that the non-French speaking candidate will strive to learn the basics of French in order to facilitate communication and integration into the laboratory.