Missions

Structure characterization of enzymes involved in glycosaminoglycan biosynthesis and training of PhD students to conduct cryo-EM experiments.

Activités

1) Structure characterization
- purification of recombinant proteins
- cryo-EM sample preparation and data collection
- data processing, interpretation and model building

2) Training and supervision
- teach cryo-EM workflow to PhD students in the team
- project coordination

3) Manuscript and grant writing
- writing and proof-reading of scientific articles
- assisting in grant and report writing

Compétences

1) Basic knowledge
- Strong understanding of enzymatic reaction mechanisms and protein structure-function relationships.
- Familiarity with different types of glycosylation found in cells.
- English language proficiency at level C1.
- At least three first-author publications in peer-reviewed journals

2) Technical know-how
- Proficient in Cryo-EM grid preparation, data collection, and processing.
-Experienced in protein purification using FPLC.
-Skilled in working with mammalian cells under sterile conditions


3) Behavioural and relational skills
-Able to work effectively in a team-oriented environment
-Excellent organizational skills for managing research projects
- Creativity, responsibility and solution-oriented

Contexte de travail

The IBS (300 people) is a joint CEA-CNRS-UGA research unit located in the scientific environment of the Grenoble presqu'ile, which houses CEA and CNRS units, as well as the European laboratories of the ILL, ESRF and EMBL. This unique environment offers exceptional infrastructure for pursuing challenging structural biology projects, along with a vibrant scientific community.
The SAGAG group (Structure and Activity of Glycosaminoglycans group) is composed of three teams and has a long-standing, internationally recognized expertise in characterizing heparan sulfate structures and their interaction with proteins. The Wild team focusses on the functional and structural characterization of enzymes involved in glycosaminoglycan biosynthesis and the postdoc candidate will be expected to work closely together with other group members to advance and complete ongoing projects.
The team has direct access to several platforms and facilities, including a well-equipped electron microscopy facility, protein crystallization, mass-spectrometry and several instruments for the biophysical characterization of proteins. We benefit from regular access to a 200kV Glacios and 300kV Titan Krios TEM for grid screening and data collection.
You can find more information here:
https://www.ibs.fr/en/research/membrane-proteins-and-glycobiology/structure-and-activity-of-glycosaminoglycans-group-h-lortat-jacob/wild-team?lang=en

Contraintes et risques

Without.
The research will be carried out in either French or English, however it is expected that the non-French speaking candidate will strive to learn the basics of French in order to facilitate communication and integration into the laboratory.