Reference : UMR5075-CYNDAV-030
Workplace : GRENOBLE
Date of publication : Wednesday, June 22, 2022
Type of Contract : FTC Scientist
Contract Period : 24 months
Expected date of employment : 1 October 2022
Proportion of work : Full time
Remuneration : between €2663 gross per month and €3783 gross per month depending on experience
Desired level of education : PhD
Experience required : 1 to 4 years
Kinetic and structural study of the maturation of a new generation antibiotic activated by the presence of beta-lactamase
Expression and purification of the enzymes used to activate the antibiotic (beta-lactamases) as well as the enzymes targeted by the antibiotic in its activated form (PBP or LDt).
Measurement of their enzymatic activity
Reactional study of the activation processes of the antibiotic as well as the inhibition of the target
Structural study of enzyme-substrate interactions by NMR and crystallography
In situ study of pro-drug activation and target inhibition in bacteria
Training in chemistry, biochemistry, structural biology
Experience in biochemistry, microbiology and structural biology
IBS (300 people) is a joint CEA-CNRS-UGA research unit located on the Grenoble scientific campus, which brings together CEA and CNRS laboratories, as well as the European structures of the ILL, ESRF and EMBL, creating a dynamic local synergy which is reflected in strong partnerships (Partnership for Structural Biology - PSB)
You will work in the NMR group under the supervision of the "NASPEC" project leader of the call for projects on antibiotic resistance, bringing together several teams on the development of narrow-spectrum antibiotics to combat the emergence of bacterial resistance.
Translated with www.DeepL.com/Translator (free version)
Constraints and risks
Risks inherent to handling in a classic P1 biochemistry laboratory.
Use of NMR equipment requiring that the equipment only be approached in the absence of a "pacemaker" type health device.
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