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Portail > Offres > Offre UMR5075-CYNDAV-008 - Chercheur en cryo-microscopie électronique et radiocristallographie (H/F)

Researcher in cryo-electron microscopy and radiocrystallography (H/F)

This offer is available in the following languages:
Français - Anglais

Date Limite Candidature : lundi 26 octobre 2020

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General information

Reference : UMR5075-CYNDAV-008
Workplace : GRENOBLE,GRENOBLE
Date of publication : Monday, October 05, 2020
Type of Contract : FTC Scientist
Contract Period : 12 months
Expected date of employment : 1 December 2020
Proportion of work : Full time
Remuneration : 2648€
Desired level of education : PhD
Experience required : 1 to 4 years

Missions

The successful candidate will study bacterial stress response using cryo-electron microscopy and X-ray crystallography

Activities

Sample preparation and optimization for quality control by
negative stain EM followed by observation and high-resolution data
acquisition by cryo-EM. Cryo-EM image processing. Preparation and
optimisation of protein crystals and their analysis by X-ray crystallography.
Biochemical characterisation of the proteins of interest.

Skills

Sample preparation for EM. Grid preparation for high resolution cryo-EM data acquisition. Operation of the electron microscopes of the IBS Platform (T12, F20, Glacios) autonomously, within the defined health and safety rules, or strong will to learn their operation. Proficiency in using the informatics tools necessary for image processing (Linux, computing cluster, image processing software). Experience in X-ray crystallography and biochemistry.
Good oral and written presentation skills, writing regular reports on achieved experiments and contributing to the writing of scientific articles.
Ability to work in an international and pluridisciplinary team, independent thinking, rigor and organization skills, participation to general laboratory tasks, willigness to transmit experience and passion, take on responsibilities.
English level B2 minimum in comprehension and oral.

Work Context

The IBS is a research institute affiliated to the Université Grenoble-Alpes, the CNRS and the CEA. It currently employs 280 scientists, including many students and post-docs from all over the world. It enjoys access to high-end infrastructure and equipments in cellular and molecular biology, biochemistry, biophysics and structural biology. IBS is one of the founders of the Partnership for Structural Biology (PSB) with prestigious european institutes : the European Synchrotron Radiation Facility (ESRF), the ILL neutron scattering facilities, and the European Molecular Biology Laboratory (EMBL) Grenoble outstation, all situated in the vicinity providing a strong international environement.
Within the IBS, the successful candidate will join the group of Dr. Irina Gutsche, “Microscopic Imaging of Complex Assemblies” (MICA).

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