Missions

The post-doctoral researcher will be responsible for the phenotypic and functional characterization of organoids derived from human bone marrow stromal cells. This project is part of a collaborative project with other partners (Inserm), and our laboratory is responsible for the production and phenotypic and functional study of organoids for the consortium. The candidate will be in charge of these studies and those on native tissue. Research will be carried out in collaboration with the Institute's teams, associated laboratories and technology platforms. Finally, he/she will co-supervise students (M2 and young PhD students).

Activities

The candidate will produce organoids in 3D culture from human bone marrow mesenchymal stromal cells from aged or young donors. Senescent cells will be produced and added to the system to artificially produce aged organoids for phenotypic and functional studies. Phenotypic studies will be carried out using microscopy (confocal, immunohistochemistry, 3D microscopy), flow cytometry, biochemistry (Western blot) and molecular biology (qRT-PCR) techniques. Functional studies will be carried out in vitro, assessing the cells' potential for differentiation, proliferation and self-organization.

Skills

Expertise in 2D and 3D cell culture. Biochemical techniques for detecting molecules, such as Western blot, QRT-PCR and ELISA. Techniques for visualizing cells and tissues using confocal microscopy, immunohistochemistry and flow cytometry. Excellent written and spoken technical English. Intellectual curiosity, willingness to learn and work in a group. Self-motivated. Ability to interpret/analyse results and have a synthetic mind. Ability to supervise students at different levels.

Work Context

30-month post-doctoral position (1 to 3 years' experience preferred) on an ANR-funded research project in Team 4 GOT-it of the Restore Institute. This institute is a research unit (Inserm, CNRS, EFS-Occitanie, Univ. Toulouse 3, ENVT). The institute brings together over a hundred people in 5 teams, whose aim is to develop integrated, multi-disciplinary research (cell biology, physiology, cell and tissue engineering, bioinformatics, mathematics, biophysics, machine learning, AI), in support of minimally invasive, targeted preventive and predictive medicine capable of limiting age-related functional disorders. Team 4 conducts research (and development) on organoids mimicking tissues such as bone marrow and subcutaneous adipose tissue.

Constraints and risks

Hepatitis B vaccination required