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Portail > Offres > Offre UMR5048-FLOLEP-050 - Ingénieur en Biophysique H/F

Biophysics engineer position M/F

This offer is available in the following languages:
Français - Anglais

Date Limite Candidature : lundi 13 février 2023

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General information

Reference : UMR5048-FLOLEP-050
Nombre de Postes : 1
Date of publication : Monday, January 23, 2023
Type of Contract : FTC Technical / Administrative
Contract Period : 12 months
Expected date of employment : 1 April 2023
Proportion of work : Full time
Remuneration : Between 2236 € and 2358 € according to experience
Desired level of education : 3-year university degree
Experience required : 1 to 4 years


We are looking for a highly motivated candidate to fill a French National Research Agency (ANR)/European Research Council (ERC)-funded engineer position to work on the optimization and use of this RNA-FISH method to investigate motility mechanisms in bacterial biofilms. .


The candidate will be in charge of:
- Designing and amplifying FISH probe libraries,
- Growing bacterial colonies,
- Performing RNA-FISH, and
- Acquiring multiplexed imaging datasets.


The candidate will have expertise in molecular biology.
The candidate is expected to express a strong interest in working at the interface between biology, bioinformatics, and physics.

Work Context

The Center for Structural Biology (CBS) carries out research at the forefront of structural biology and biophysics as a means to reveal the fundamental physical mechanisms underlying biological activity and its regulation. The CBS is located at the heart of a world-class research campus in Biology in the vibrant city of Montpellier in the French Riviera. It hosts research groups with expertise in many advanced microscopies, including multiplexed, high-throughput microscopy, single-molecule super-resolution (PALM/STORM), 3D structured illumination microscopy, fluorescence correlation spectroscopies, magnetic and optical tweezers, high-speed atomic force microscopy and cryo-electron microscopy.

Our interdisciplinary group is interested in understanding the mechanisms underlying DNA organization and transcription in multicellular organisms, including bacterial biofilms. Because the transcriptional program of individual bacteria is highly heterogeneous during biofilm development, traditional sequencing-based technologies are ill-suited to decorticate the differentiation program of subpopulations of cells. To circumvent this limitation, our lab recently developed a sequential version of RNA FISH to detect RNA genes in single cells in situ without disrupting the spatial organization of the bacterial colony.

Constraints and risks

The contract can start as early as 1 April 2023 (1 year with the possibility of renewal).
Minimum time of experience requested: none.
Interested applicants should deposit their CV with references.

Additional Information

Please visit our website for further details: http://nollmannlab.org/

Relevant Publications
Rombouts et al, BioRxiv 2022.
Espinola, Goetz, et al. Nature Genetics, 2021, 53, pages 477-486.
Guilhas, et al. Mol Cell, 2020 Jul 16;79(2):293-303.e4.
Cardozo Gizzi, et al., Mol Cell, 2019, 74, Issue 1, p1-222.
Faure, Fiche et al., Nature, 2016, 539(7630):530-535.
Marbouty, Le Gall, et al. Mol Cell, 2015, 59 (4): 588-602.

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