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Portail > Offres > Offre UMR5048-FLOLEP-044 - Post-doctorat en analyse structurale LLPS (H/F)

Post-doctoral position on LLPS Structural Analysis

This offer is available in the following languages:
Français - Anglais

Date Limite Candidature : jeudi 8 décembre 2022

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General information

Reference : UMR5048-FLOLEP-044
Workplace : MONTPELLIER
Date of publication : Thursday, November 17, 2022
Type of Contract : FTC Scientist
Contract Period : 18 months
Expected date of employment : 1 February 2023
Proportion of work : Full time
Remuneration : Between 3257€ and 4003€ monthly gross according to experience
Desired level of education : PhD
Experience required : 1 to 4 years

Missions

Our group at the Center for Structural Biology (CBS) at the University of Montpellier (South of France) offers one open Postdoc position (18 months) to work on lipid-lipid phase separation (LLPS), a new paradigm in cell compartmentalization, using atomic force microscopy (AFM) including high-speed modality (see ref.1) and correlative AFM-fluorescence microscopy (see ref.2-3).

Activities

The project aims at analyzing the formation of LLPS condensates of TopBP1 in vitro and/or in cellulo. TopBP1 (Topoisomerase IIß-binding protein) is a hub protein in human cells that functions at the crossroad of multiple vital cellular processes and assembles nuclear condensates to switch on ATR signaling (see ref.4).

Skills

The project is highly interdisciplinary, at the interface of biophysics, cell biology and biochemistry. Therefore, successful applicants are expected to have training and research experience in physics/biophysics, especially advanced skills in AFM. Previous experience in fluorescence imaging (confocal/TIRF/Super Resolution/FLIM), Optics as well as biochemistry, cell biology and computation (e.g. Python) skills will be positively considered. The candidates should have a solid publication record (at least one first author paper). Applicants are expected to have defended their PhD thesis within the last 2 years (< 2 years postdoctoral experience).

Work Context

We are an international team at an interdisciplinary research center with excellent in-house access to state-of-the-art biophysics infrastructure and biochemistry and cell culture facilities. Moreover, several groups at the CBS are working on LLPS, which fosters creativity and collaborations.
The research of PE Milhiet's group focuses on the development and use of advanced microscopies (AFM and single molecule fluorescence microscopy) to decipher the molecular mechanisms associated to the organization and remodeling of biological membranes. Our team is pluridisciplinary and counts 15 people including 8 permanent researchers.
CBS is an Institute dedicated to research at the forefront of structural biology, biophysics and Bioengineering. It also proposes facilities in these fields. Montpellier is a stunning city on the French Riviera with a significant international research community.

Constraints and risks

Interested applicants should deposit their CV, cover letter and references.

Additional Information

References
1) Nasrallah H. et al. J Mol Biol 1886:45, 2019. 2) A. Vial et al. Nanoscale, 13(29):12484, 2021. 3) T. Fernandes et al. Scientific Reports, 10(1):7098, 2020. 4) Frattini C et al. Mol Cell. 81(6):1231, 2021.

For more information:
"Integrative Biophysics of Membrane" group
https://integrativebiophysicsofmembranes.wordpress.com/
https://twitter.com/MembranesBiophy

We talk about it on Twitter!