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Portal > Offres > Offre UMR5031-NICMAN-002 - Evolution dirigée d'enzymes par microfluidique pour des biopiles glucose/O2 (H/F)

Directed evolution of enzymes for biofuel cells applications by microfluidics

This offer is available in the following languages:
Français - Anglais

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General information

Reference : UMR5031-NICMAN-002
Workplace : PESSAC
Date of publication : Wednesday, November 06, 2019
Type of Contract : FTC Scientist
Contract Period : 15 months
Expected date of employment : 3 February 2020
Proportion of work : Full time
Remuneration : 2617 € before taxes
Desired level of education : PhD
Experience required : Indifferent

Missions

As part of an ANR project, we are offering a 15-months post doc position at the Paul Pascal Research Center (CRPP) located in Bordeaux (France). The main goal is the screening of enzymes by microfluidic to design efficient enzymatic glucose/O2 biofuel cell. For this, the candidate will need to create DNA libraries for expression of enzymes in bacteria or yeasts that will be subsequently screened by microfluidics. After sequencing and selection, the most promising mutants will be produced and then tested electrochemically. This interdisciplinary project is based on the combination of the expertise of two CRPP teams. The candidate will work under the direction of Dr. Nicolas Mano (team Biopiles and Biocapteurs) and Prof. Jean-Christophe Baret (Soft Micro Systems team). The Biopiles and Biosensors team is interested in biolectrochemistry and enzymatic engineering. The Soft Micro Systems Group is interested in the application of microfluidic technologies in biotechnology and synthetic biology

Activities

Molecular Biology
Enzymology
Microfluidic
Electrochemsitry

Skills

The candidate must hold a Ph.D. in Molecular Biology / Enzymology obtained less than two years ago and will have experience in DNA libraries and screening. Skills in microfluidic will be highly appreciated. We are looking for highly motivated candidates, showing a strong interest in multi-disciplinary projects. The candidate must be rigorous, have excellent organizational and teamwork skills, have the ability to lead, and be able to express himself /herself in English; written and spoken.

Work Context

The Paul Pascal Research Center (CRRP- http://www.crpp-bordeaux.cnrs.fr/) is widely recognized for the development and characterization of complex systems, the laboratory covers diverse areas of research from soft matter to advanced materials to the interface of physical chemistry and biology.

Additional Information

Required documents: A CV including the list of publications, a letter of motivation and two references that may be contacted

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