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Portail > Offres > Offre FR550-BRUMIR-013 - Post-doctorat "Etudes des interactions protéine-proteine dans le métabolome purinosome" (H/F)

Post-doc "Protein-protein interactions in the purinosome metabolon" (M/F)

This offer is available in the following languages:
Français - Anglais

Date Limite Candidature : vendredi 29 octobre 2021

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General information

Reference : FR550-BRUMIR-013
Workplace : PARIS 05
Date of publication : Friday, September 17, 2021
Type of Contract : FTC Scientist
Contract Period : 24 months
Expected date of employment : 1 January 2022
Proportion of work : Full time
Remuneration : Gross salary will vary with candidate's experience starting at 3106€/month before taxes (around 2500€ after taxes) for candidate with less than 2 years post-doctoral experience. See the official CNRS salary table for researcher at http://labexdynamo.ibpc.fr/fp-dynamo-paris/
Desired level of education : PhD
Experience required : Indifferent

Missions

Attention: You must not have resided or carried out your main activity (e.g. work or studies) in France for more than 12 months in the 3 years prior to the recruitment date.
You have research experience in the field relevant to the call.

The goal of the project is to investigate by multi-scale computer simulations the structure, dynamics and ultimately function of a metabolon: the purinosome. The aim is to gain molecular insights into the control of the metabolic flux and its regulation in the purinosome, and possibly unravel novel therapeutic strategies. The computational investigation will be based on the innovative technique developed in the host laboratory, the lattice Boltzmann Molecular Dynamics, based on coarse-grained models for proteins combined to atomistic simulations.
PI: Fabio Sterpone, UPR9080, sterpone@ibpc.fr

Publications related to the project :
An, S., Kumar, R., Sheets, E.D., Benkovic, S.J., 2008. Reversible Compartmentalization of de novo Purine Biosynthetic Complexes in Living Cells. Science 320, 103–106
Sterpone, F., Derreumaux, P., Melchionna, S., 2015. Protein simulations in fluids: coupling the OPEP coarse-grained force field with hydrodynamics. J. Chem. Theory Comput. 11, 1843-1853.
Sterpone F., et al. 2014. The OPEP coarse-grained protein model: from single molecules, amyloid formation, role of macromolecular crowding and hydrodynamics to RNA/DNA complexes. Chem. Soc. Rev. 43, 4871-4893.
Timr, S., Gnutt, D., Ebbinghaus, S., Sterpone, F., 2020. The Unfolding Journey of Superoxide Dismutase 1 Barrels under Crowding: Atomistic Simulations Shed Light on Intermediate States and Their Interactions with Crowders, J. Phys. Chem. Lett. 11, 4206-421.
Timr, S., Sterpone, F., 2021. Stabilizing or Destabilizing: Simulations of Chymotrypsin Inhibitor 2 under Crowding Reveal Existence of a Crossover Temperature, J. Phys. Chem. Lett. 12, 1741-1746.
Gnutt, D., Timr, S., Ahlers, J., König, B., Manderfeld, E., Heyden, M., Sterpone, F., Ebbinghaus, S., 2019b. Stability Effect of Quinary Interactions Reversed by Single Point Mutations. J. Am. Chem. Soc.

Activities

- Perform multi-scale coarse-grained simulations of purinosome
- Perform atomistic Molecular Dynamics simulations
- Coding script for analysis
- Implement advanced algorithm to accelerate sampling

Skills

Skills :
- Background in computation physics and/or chemistry.
- Programming languages Python and other languages (Fortran, C, etch)
- Experience in simulations in parallel HPC environments

Know-how
- Writing scientific papers
- Presentation of results in form of poster and talk
- English speaking

Soft skills :
- Team work attitude

Work Context

The candidate will join the team of F. Sterpone in the LBT at IBOC. Software development, including visiting period, will be carried out in collaboration with S. Melchionna (Lexma- Technology, Rome, Italy). All computational work will be allocated in HPC resources from French national network (Genci). Experimental collaboration will be done with S. Ebbinghaus (TU Braunschweig, Germany), this will include some visiting.

The COFUND FP-DYNAMO-PARIS programme offers a unique interdisciplinary environment and a fundamental research initiative in the centre of Paris to train the scientists of tomorrow in the field of physico-chemical biology. The goal of the research programme is to integrate knowledge on gene expression, structural and membrane biology, and bioenergetics in bacteria, chloroplasts and mitochondria in the general context of improving our understanding the biogenesis of energy-transducing membranes. These studies will involve cutting-edge technologies including structural biology (NMR, X-ray crystallography and CryoEM), RNA sequencing, mass-spectrometry, synthetic biology, microfluidics, computational modelling and visualization. Post-doctoral fellows will have the opportunity of developing their research projects in a dynamic scientific environment covering three main research themes: i) RNA biology from bacteria to chloroplasts, ii) Membrane system dynamics and iii) computational modelling of molecular assemblies. They will benefit from the supervision of internationally-recognised experts in their fields, a world-wide network of renowned institutions, cutting-edge research infrastructure and state-of-the-art technical know-how that will allow them to hone their scientific skills, make themselves known and increase their competitiveness on the labour market. Specific training will be proposed to ERs through attendance of the EMBO course on “Laboratory Leadership for Postdocs” included in a retreat in Padua, and involvement in dedicated complementary soft skill training events.

Constraints and risks

The contract must start between 1 January 2022 and 31 May.

Additional Information

The application should be written in English and uploaded at https://emploi.cnrs.fr/. It should include a detailed CV, a minimum of two letters of reference, a letter of motivation describing your short and long term career objectives, and a signed declaration stating that you are in compliance with the mobility rule which stipulates that you have not resided or carried out your main activity (work or studies) in France for more than 12 months during the three years preceding the date of recruitment. In addition, the complete file must also be sent to Bruno MIROUX & Magdalena TORTYNA, respectively director and manager of the project at the following email address DYNAMOcofund@ibpc.fr (Tel: 33 1 58 41 50 64). See details on the website http://labexdynamo.ibpc.fr/fp-dynamo-paris/
The position is intended as an initial step in a research career, and the assessment of the applicants will primarily be based on their research qualifications and their potential as researchers with skills that complement and strengthen on-going research within the partner institutions.
Step 1. The applications of each eligible candidate will be evaluated by independent international experts within the relevant research theme. Eligible applicants will be evaluated based on merits, motivation for the program, academic excellence and personal qualifications such as collaborative and communication skills.
Step 2. A maximum of three candidates will be retained for a video interview with the independent experts for each open position (Nov 2021). After the interview, candidates on the short list will be invited to a conference call with the project supervisor and a DYNAMO board member to define the general scope of the project, the training and the research environment. Candidates will be asked to draft a 3-page project proposal, bringing their own original ideas and will be given 2 weeks to submit it.
Step 3. Each project proposal will be evaluated by one of the independent experts and the DYNAMO board member who evaluated the candidate in the previous steps. The final selection will be made based on the scores obtained at steps 1, 2 and 3. The minimum score must be above 20/40. Top candidate will be offered the postdoc position with a starting date from January 1- May 31, 2022.

The CNRS (Centre National de la Recherche Scientifique) is an equal opportunity employer. Furthermore, FP-DYNAMO-PARIS is committed to promoting the role of women in science and thus explicitly encourages women to apply.

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