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Portail > Offres > Offre FR550-BRUMIR-011 - Post-doctorat "Biogénèse membranaire dans le modèle AtpF chez E. coli et Chlamydomonas reinhardtii." (H/F)

Postdoc AtpF-mediated membrane biogenesis in E. coli and C. reinhardtii (M/F)

This offer is available in the following languages:
Français - Anglais

Date Limite Candidature : vendredi 29 octobre 2021

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General information

Reference : FR550-BRUMIR-011
Workplace : PARIS 05
Date of publication : Friday, September 17, 2021
Type of Contract : FTC Scientist
Contract Period : 24 months
Expected date of employment : 1 January 2022
Proportion of work : Full time
Remuneration : Gross salary will vary with candidate's experience starting at 3106€/month before taxes (around 2500€ after taxes) for candidate with less than 2 years post-doctoral experience. See the official CNRS salary table for researcher at http://labexdynamo.ibpc.fr/fp-dynamo-paris/
Desired level of education : PhD
Experience required : Indifferent

Missions

Attention: You must not have resided or carried out your main activity (e.g. work or studies) in France for more than 12 months in the 3 years prior to the recruitment date.
You have research experience in the field relevant to the call.

Supervision : Francesca Zito zito@ibpc.fr et Bruno Miroux, miroux@ibpc.fr UMR7099 Laboratory of Physical and Chemical Biology of Membrane Proteins
Collaborations : Christophe Tribet P.A.S.T.E.U.R. de l'ENS-Paris ; TEM facilitees Padoue, Italy and NanoImagine platform, Pasteur Institute, Paris, France
The “revolution” in the resolution of cryo-electron microscopy opens new avenues to observe membrane proteins (MPs) in native membranes (1). Our lab contributed in the cryo-electron microscopy analysis of MPs by developing alternative surfactant named amphipol and by the genetic improvement of bacterial expression hosts (2, 3). Here we propose to study membrane biogenesis in the “AtpF model”. The overproduction of the b subunit of the FoF1-ATP synthase, AtpF, induces massive intracellular membrane (ICM) proliferation in C43(DE3) host (4), and preliminary results indicate that the expression of AtpF in the chloroplast of C reinhardtii might also shape its thylakoids membrane organization. Depending of its motivation and competences, the FP-DYNAMO fellow will have the opportunity to develop its project in several directions: i tomography on both models, Chlamydomonas chloroplast is a perfect material to study the thylakoids architecture by cryo-microscopy; ii, omic approaches; iii, genetics, by selective disruption of candidate genes involved in membrane biogenesis; iv, biochemical and synthetic biology approaches. Our long-term project is to contribute to elucidate basic molecular mechanism of membrane proliferation across evolution and to apply them for the construction of a synthetic membrane organelle (5).

Publications
1. Le Bon C, Michon B, Popot J-L, Zoonens M. 2021. Amphipathic environments for determining the structure of membrane proteins by single-particle electron cryo-microscopy. Q Rev Biophys 54:e6.
2. Angius F, Ilioaia O, Amrani A, Suisse A, Rosset L, Legrand A, Abou-Hamdan A, Uzan M, Zito F, Miroux B. 2018. A novel regulation mechanism of the T7 RNA polymerase based expression system improves overproduction and folding of membrane proteins. Sci Rep 8:8572.
3. Dilworth MV, Piel MS, Bettaney KE, Ma P, Luo J, Sharples D, Poyner DR, Gross SR, Moncoq K, J F Henderson P, Miroux B, Bill RM. 2018. Microbial expression systems for membrane proteins. Methods San Diego Calif 147:3–39.
4. Carranza G, Angius F, Ilioaia O, Solgadi A, Miroux B, Arechaga I. 2017. Cardiolipin plays an essential role in the formation of intracellular membranes in Escherichia coli. Biochim Biophys Acta 1859:1124–1132.
5. Royes J, Ilioaia O, Lubart Q, Angius F, Dubacheva GV, Bally M, Miroux B, Tribet C. 2019. Bacteria-Based Production of Thiol-Clickable, Genetically Encoded Lipid Nanovesicles. Angew Chem Int Ed Engl 58:7395–7399.

Activities

- analyse the morphology and quantify membrane proliferation in E. coli and Chlamydomonas reinhardtii
- decipher the genetic determinants of membrane proliferation in E. coli and Chlamydomonas reinhardtii
- develop applications of vectorised and/or functionalized nanovesicles
- write scientific articles for international peer-reviewed journals

Skills

knowledge
- General knowledge in biology and microbiology
- In-depth knowledge of biochemistry and membrane systems
- Knowledge of molecular biology
- Proficiency in scientific English (reading and writing)
- Basic knowledge of digital data processing
- Previous experience in microscopy and/or proteomics will be a plus
Know-how
- Design and implement experimental protocols
- Ability to analyse and report information (keeping a laboratory notebook, preparing summary tables of results, preparing oral presentations).
- Prepare articles for publication in international peer-reviewed journals
- Carry out quantitative and statistical analyses of batches of microscopy images and data collected.
Skills
- work in a team, especially in multi-laboratory collaboration
- organise and plan your work independently;
- present results in the form of clear and detailed scientific reports.

Work Context

Most of the work will be carried out in the UMR7099 "Laboratoire de Biologie Physico-Chimique des protéines Membranaires", directed by Bruno Miroux at the Institut de Biologie Physico-Chimique in Paris http://www.ibpc.fr/UMR7099. This laboratory, located in the heart of the Latin Quarter, has a staff of just over 25, including 19 statutory members. The project will be carried out under the direction of Francesca Zito and Bruno Miroux.
The COFUND FP-DYNAMO-PARIS programme offers a unique interdisciplinary environment and a fundamental research initiative in the centre of Paris to train the scientists of tomorrow in the field of physico-chemical biology. The goal of the research programme is to integrate knowledge on gene expression, structural and membrane biology, and bioenergetics in bacteria, chloroplasts and mitochondria in the general context of improving our understanding the biogenesis of energy-transducing membranes. These studies will involve cutting-edge technologies including structural biology (NMR, X-ray crystallography and CryoEM), RNA sequencing, mass-spectrometry, synthetic biology, microfluidics, computational modelling and visualization. Post-doctoral fellows will have the opportunity of developing their research projects in a dynamic scientific environment covering three main research themes: i) RNA biology from bacteria to chloroplasts, ii) Membrane system dynamics and iii) computational modelling of molecular assemblies. They will benefit from the supervision of internationally-recognised experts in their fields, a world-wide network of renowned institutions, cutting-edge research infrastructure and state-of-the-art technical know-how that will allow them to hone their scientific skills, make themselves known and increase their competitiveness on the labour market. Specific training will be proposed to ERs through attendance of the EMBO course on “Laboratory Leadership for Postdocs” included in a retreat in Padua, and involvement in dedicated complementary soft skill training events.

Constraints and risks

The contract must start between 1 January 2022 and 31 May.

Additional Information

The application should be written in English and uploaded at https://emploi.cnrs.fr/. It should include a detailed CV, a minimum of two letters of reference, a letter of motivation describing your short and long term career objectives, and a signed declaration stating that you are in compliance with the mobility rule which stipulates that you have not resided or carried out your main activity (work or studies) in France for more than 12 months during the three years preceding the date of recruitment. In addition, the complete file must also be sent to Bruno MIROUX & Magdalena TORTYNA, respectively director and manager of the project at the following email address DYNAMOcofund@ibpc.fr (Tel: 33 1 58 41 50 64). See details on the website http://labexdynamo.ibpc.fr/fp-dynamo-paris/
The position is intended as an initial step in a research career, and the assessment of the applicants will primarily be based on their research qualifications and their potential as researchers with skills that complement and strengthen on-going research within the partner institutions.
Step 1. The applications of each eligible candidate will be evaluated by independent international experts within the relevant research theme. Eligible applicants will be evaluated based on merits, motivation for the program, academic excellence and personal qualifications such as collaborative and communication skills.
Step 2. A maximum of three candidates will be retained for a video interview with the independent experts for each open position (Nov 2021). After the interview, candidates on the short list will be invited to a conference call with the project supervisor and a DYNAMO board member to define the general scope of the project, the training and the research environment. Candidates will be asked to draft a 3-page project proposal, bringing their own original ideas and will be given 2 weeks to submit it.
Step 3. Each project proposal will be evaluated by one of the independent experts and the DYNAMO board member who evaluated the candidate in the previous steps. The final selection will be made based on the scores obtained at steps 1, 2 and 3. The minimum score must be above 20/40. Top candidate will be offered the postdoc position with a starting date from January 1-May 31, 2022.

The CNRS (Centre National de la Recherche Scientifique) is an equal opportunity employer. Furthermore, FP-DYNAMO-PARIS is committed to promoting the role of women in science and thus explicitly encourages women to apply.

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