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Portail > Offres > Offre FR550-BRUMIR-008 - Post-doctorat "Etude de l'espace énergétique de récepteurs couplés aux proteines G" (H/F)

Post-doc "Study of the energy landscape of G Protein-Coupled Receptors" (M/F)

This offer is available in the following languages:
Français - Anglais

Date Limite Candidature : jeudi 28 octobre 2021

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General information

Reference : FR550-BRUMIR-008
Workplace : PARIS 05
Date of publication : Friday, September 17, 2021
Type of Contract : FTC Scientist
Contract Period : 24 months
Expected date of employment : 1 January 2022
Proportion of work : Full time
Remuneration : Gross salaries will vary with candidate's experience starting at 3106€/month before taxes for candidate with less than 2 years post-doctoral experience.
Desired level of education : PhD
Experience required : Indifferent

Missions

Attention: You must not have resided or carried out your main activity (e.g. work or studies) in France for more than 12 months in the 3 years prior to the recruitment date.
You have research experience in the field relevant to the call.

Most hormones and neurotransmitters communicate information to cells via G protein coupled receptors (GPCRs). The large number of biological functions they control also makes these membrane receptors one of the most important families of pharmacological targets in biomedicine. GPCRs are considered to be flexible and dynamic proteins per se, i.e. they can visit several conformational states linked to distinct biological outcomes. Despite a very large number of structures at the atomic scale that are regularly published, these data must be completed to fully understand the function of these complex allosteric machines. The project aims at characterizing the energy landscape of these receptors in complement to low-energy and highly populated published atomic structures by advanced biophysical methods: NMR (kinetic barriers and conformational ensembles), electron cryo-microscopy (structural aspects & conformational ensembles) and single molecule force spectroscopy (kinetics of interactions between ligands and/or effectors with receptors).
PI: Laurent Catoire, UMR7099, catoire@ibpc.fr, Co-PI : Karine Moncoq, UMR7099, moncoq@ibpc.fr

Publications related to project:
- Louet et al. (2021) Concerted conformational dynamics and water movements in the ghrelin G protein-coupled receptor. Elife, 10, e63201.
- Damian et al. (2021) Allosteric modulation of ghrelin receptor signaling by lipids. Nat Commun. 12(1):3938. doi: 10.1038/s41467-021-23756-y
- Giusti et al. (2020) Structure of the agonist 12-HHT in its BLT2 receptor-bound state. Sci Rep. 10(1):2630. doi: 10.1038/s41598-020-59571-6.
- Casiraghi et al. (2019) NMR analysis of GPCR conformational landscapes and dynamics. Mol Cell Endocrinol. 484:69-77. doi: 10.1016/j.mce.2018.12.019.
- Casiraghi et al. (2018) Illuminating the Energy Landscape of GPCRs: The Key Contribution of Solution-State NMR Associated with Escherichia coli as an Expression Host. Biochemistry 57(16):2297-2307. doi: 10.1021/acs.biochem.8b00035.
- Chipot et al. (2018) Perturbations of Native Membrane Protein Structure in Alkyl Phosphocholine Detergents: A Critical Assessment of NMR and Biophysical Studies. Chem Rev. 118(7):3559-3607. doi: 10.1021/acs.chemrev.7b00570.
- Casiraghi et al. (2016) Functional Modulation of a G Protein-Coupled Receptor Conformational Landscape in a Lipid Bilayer. J Am Chem Soc. 138(35):11170-5. doi: 10.1021/jacs.6b04432.

Activities

- productions and purifications of receptors
- productions and purifications of lipoproteins
- incorporation of réceptors in lipid nanodiscs
- sample preparations for different biophysical techniques (RMN, cryoEM, force spectroscopy)
- biophysical studies

Skills

Knowledge:
- biochemistry of membrane proteins
- physico-chimistry of lipids and surfactants
- knowledge of one of the following biophysical techniques will be appreciated: NMR or cryoEM or single molecule force spectroscopy
- scientific English (spoken, reading and writing)

Know-how :
- Design and implement of experimental protocols
- Ability to analyze and restore information (keeping a laboratory notebook, preparing summary tables of results, preparing oral presentations)
- Writing articles for publication in international peer-reviewed journals

Work within a common structure :
- work in a team, especially in a multi-laboratory collaboration
- participation in laboratory management (inventory, orders, cleanliness, respect for common equipment, etc.)
- organize and plan their work independently
- present its results in the form of clear and detailed scientific reports

Work Context

Most of the work will be carried out at the Laboratory of Biology and Physico-Chemistry of Membrane Proteins, UMR 7099 CNRS, University of Paris, located at the Institute of Physico-Chemistry Biology (IBPC) in Paris (13, rue Pierre et Marie Curie , 75005, Paris, France).
The COFUND FP-DYNAMO-PARIS programme offers a unique interdisciplinary environment and a fundamental research initiative in the centre of Paris to train the scientists of tomorrow in the field of physico-chemical biology. The goal of the research programme is to integrate knowledge on gene expression, structural and membrane biology, and bioenergetics in bacteria, chloroplasts and mitochondria in the general context of improving our understanding the biogenesis of energy-transducing membranes. These studies will involve cutting-edge technologies including structural biology (NMR, X-ray crystallography and CryoEM), RNA sequencing, mass-spectrometry, synthetic biology, microfluidics, computational modelling and visualization. Post-doctoral fellows will have the opportunity of developing their research projects in a dynamic scientific environment covering three main research themes: i) RNA biology from bacteria to chloroplasts, ii) Membrane system dynamics and iii) computational modelling of molecular assemblies. They will benefit from the supervision of internationally-recognised experts in their fields, a world-wide network of renowned institutions, cutting-edge research infrastructure and state-of-the-art technical know-how that will allow them to hone their scientific skills, make themselves known and increase their competitiveness on the labour market. Specific training will be proposed to ERs through attendance of the EMBO course on “Laboratory Leadership for Postdocs” included in a retreat in Padua, and involvement in dedicated complementary soft skill training events.

Constraints and risks

The contract must start between 1 January 2022 and 31 May.

Additional Information

The application should be written in English and uploaded at https://emploi.cnrs.fr/. It should include a detailed CV, a minimum of two letters of reference, a letter of motivation describing your short and long term career objectives, and a signed declaration stating that you are in compliance with the mobility rule which stipulates that you have not resided or carried out your main activity (work or studies) in France for more than 12 months during the three years preceding the date of recruitment. In addition, the complete file must also be sent to Bruno MIROUX & Magdalena TORTYNA, respectively director and manager of the project at the following email address DYNAMOcofund@ibpc.fr (Tel: 33 1 58 41 50 64). See details on the website http://labexdynamo.ibpc.fr/fp-dynamo-paris/
The position is intended as an initial step in a research career, and the assessment of the applicants will primarily be based on their research qualifications and their potential as researchers with skills that complement and strengthen ongoing research within the partner institutions.
Step 1. The applications of each eligible candidate will be evaluated by independent international experts within the relevant research theme. Eligible applicants will be evaluated based on merits, motivation for the program, academic excellence, and personal qualifications such as collaborative and communication skills.

Step 2. A maximum of three candidates will be retained for a video interview with the independent experts for each open position (Nov 2021). After the interview, candidates on the short list will be invited to a conference call with the project supervisor and a DYNAMO board member to define the general scope of the project, the training and the research environment. Candidates will be asked to draft a 3-page project proposal, bringing their own original ideas and will be given 2 weeks to submit it.

Step 3. Each project proposal will be evaluated by one of the independent experts and the DYNAMO board member who evaluated the candidate in the previous steps. The final selection will be made based on the scores obtained at steps 1, 2 and 3. The minimum score must be above 20/40. Top candidate will be offered the postdoc position with a starting date from January 1- May 31, 2022.

Gross salaries will vary with candidate's experience starting at 3106€/month before taxes (around 2500€ after taxes) for candidate with less than 2 years post-doctoral experience. See CNRS salary table for researcher at: http://labexdynamo.ibpc.fr/fp-dynamo-paris/

The CNRS (Centre National de la Recherche Scientifique) is an equal opportunity employer. Furthermore, FP-DYNAMO-PARIS is committed to promoting the role of women in science and thus explicitly encourages women to apply.

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