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Portail > Offres > Offre FR550-BRUMIR-007 - Post-doctorat "Proteoliposomes à courbure thermobasculable pour la préparation de nanobioreacteurs" (H/F)

Post-doc "Flippable ELP-decorated Proteoliposomes to prepare nanobioreactors" (M/F)

This offer is available in the following languages:
Français - Anglais

Date Limite Candidature : vendredi 29 octobre 2021

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General information

Reference : FR550-BRUMIR-007
Workplace : PARIS 05
Date of publication : Friday, September 17, 2021
Type of Contract : FTC Scientist
Contract Period : 12 months
Expected date of employment : 1 January 2022
Proportion of work : Full time
Remuneration : Gross salary will vary with candidate's experience starting at 3106€/month before taxes (around 2500€ after taxes) for candidate with less than 2 years post-doctoral experience. See the official CNRS salary table for researcher at http://labexdynamo.ibpc.fr/fp-dynamo-paris/
Desired level of education : PhD
Experience required : Indifferent

Missions

Attention: You must not have resided or carried out your main activity (e.g. work or studies) in France for more than 12 months in the 3 years prior to the recruitment date.
You have research experience in the field relevant to the call.

post-doc position in Ecole Normale Superieure, UMR 8640
The project will explore a generic route to prepare biocatalytic proteoliposomes nanoreactors via the proliferation of bacterial membranes and temperature-triggered loading of chimeric enzymes. The objectives are to reconstitute and characterize biomimetic proteoliposomes by biophysical methods, including vesicle micromanipulations and optical microscopies, light scattering, microbalance. These vesicles have markedly higher loading capacities as compare to "artificial" reconstitution methods[1,2] They contain protein scaffolds with Elastic-Like Protein sequences (ELPs) that are temperature-responsive.[3] In particular, optimized sequences provided by our colleagues in the biology lab will enable to explore 1) the effect on curvature and tropism for curved areas of the ELP-fused membrane proteins, and sp. partition between flat areas and nanotubes, 2) T-switchable capture of cognate ELP-fused enzymes.
Publications related to the project:
[1, 2] A. Etemadi, et al., Artif. Cells, Nanomedicine, Biotechnol. 2016, 44, 381–391 ; T. Trantidou, et al., Engineering Compartmentalized Biomimetic Micro- and Nanocontainers, Acs Nano 11(7) (2017) 6549-6565.
[3] Royes J., Ilioaia, O.; Lubart, Q.; Angius, F.; Dubacheva, G. V.; Bally, M.; Miroux, B.; Tribet, C., Bacteria-Based Production of Thiol-Clickable, Genetically Encoded Lipid Nanovesicles. Angewandte Chemie International Edition, 2019. 58(22): p. 7395-7399.

Activities

- to prepare lipid vesicles with recombinant proteins in their membranes,
- to label vesicles and proteins with fluorophores,
- to design and mount home made devices for lipid membrane micromanipulation , nanotube extension, and optical microscopy imaging,
- to analyse the effect of thermal stress on the vesicles,
- to analyse the partition of proteins in/on the membranes,
- to write reports of results, and contribute to writing manuscripts for to publication in international journals.

Skills

skills/knowledge:
- basics in biochemistry and biophysics,
- robust background in soft matter physics and/or physical chemistry,
- background in lipid membranes and experimental physics of membranes,
- fluent english (speaking & writing)

expertise:
- lipid membrane micromanipulation,
- use to implement experiments with optical microscopes, fluorimeter/spectrophotometers, light scattering,
- statistical analysis of microscope images and implementation of mathematical models.
- team work, autonomy to organize his/her work,

Work Context

The project is conducted as a collaboration between 3 teams located in Paris 5th and 6th districts (C. Tribet's team UMR 8640, B.Miroux IBPC, UMR 7099 and S. Mangenot Univ. de Paris UMR 7057). Experimental biophysics will be done in Université de Paris, whereas chemical modifications and physical-chemistry characterization will be conducted in Ecole Normale Superieure, UMR 8640. Regularly, meetings will be hold with all partners invited to share results and discussion to coordinate the works.

The COFUND FP-DYNAMO-PARIS programme offers a unique interdisciplinary environment and a fundamental research initiative in the centre of Paris to train the scientists of tomorrow in the field of physico-chemical biology. The goal of the research programme is to integrate knowledge on gene expression, structural and membrane biology, and bioenergetics in bacteria, chloroplasts and mitochondria in the general context of improving our understanding the biogenesis of energy-transducing membranes. These studies will involve cutting-edge technologies including structural biology (NMR, X-ray crystallography and CryoEM), RNA sequencing, mass-spectrometry, synthetic biology, microfluidics, computational modelling and visualization. Post-doctoral fellows will have the opportunity of developing their research projects in a dynamic scientific environment covering three main research themes: i) RNA biology from bacteria to chloroplasts, ii) Membrane system dynamics and iii) computational modelling of molecular assemblies. They will benefit from the supervision of internationally-recognised experts in their fields, a world-wide network of renowned institutions, cutting-edge research infrastructure and state-of-the-art technical know-how that will allow them to hone their scientific skills, make themselves known and increase their competitiveness on the labour market. Specific training will be proposed to ERs through attendance of the EMBO course on “Laboratory Leadership for Postdocs” included in a retreat in Padua, and involvement in dedicated complementary soft skill training events.

Constraints and risks

Fellowship period should start between January 1 and May 31 2022.

Additional Information

The application should be written in English and uploaded at https://emploi.cnrs.fr/. It should include a detailed CV, a minimum of two letters of reference, a letter of motivation describing your short and long term career objectives, and a signed declaration stating that you are in compliance with the mobility rule which stipulates that you have not resided or carried out your main activity (work or studies) in France for more than 12 months during the three years preceding the date of recruitment. In addition, the complete file must also be sent to Bruno MIROUX & Magdalena TORTYNA, respectively director and manager of the project at the following email address DYNAMOcofund@ibpc.fr (Tel: 33 1 58 41 50 64). See details on the website http://labexdynamo.ibpc.fr/fp-dynamo-paris/
The position is intended as an initial step in a research career, and the assessment of the applicants will primarily be based on their research qualifications and their potential as researchers with skills that complement and strengthen on-going research within the partner institutions.

Step 1. The applications of each eligible candidate will be evaluated by independent international experts within the relevant research theme. Eligible applicants will be evaluated based on merits, motivation for the program, academic excellence and personal qualifications such as collaborative and communication skills.

Step 2. A maximum of three candidates will be retained for a video interview with the independent experts for each open position (Nov 2021). After the interview, candidates on the short list will be invited to a conference call with the project supervisor and a DYNAMO board member to define the general scope of the project, the training and the research environment. Candidates will be asked to draft a 3-page project proposal, bringing their own original ideas and will be given 2 weeks to submit it.

Step 3. Each project proposal will be evaluated by one of the independent experts and the DYNAMO board member who evaluated the candidate in the previous steps. The final selection will be made based on the scores obtained at steps 1, 2 and 3. The minimum score must be above 20/40. Top candidate will be offered the postdoc position with a starting date from January 1- May 31, 2022.

The CNRS (Centre National de la Recherche Scientifique) is an equal opportunity employer. Furthermore, FP-DYNAMO-PARIS is committed to promoting the role of women in science and thus explicitly encourages women to apply.

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