En poursuivant votre navigation sur ce site, vous acceptez le dépôt de cookies dans votre navigateur. (En savoir plus)
Portail > Offres > Offre FR550-BRUMIR-002 - Post-doctorat "Contrôle de l'initiation de la traduction chez les bactéries : une étude transcriptomique" (H/F)

Post-doc "Control of translation initiation in bacteria: a transcriptomic study" (M/F)

This offer is available in the following languages:
Français - Anglais

Date Limite Candidature : vendredi 29 octobre 2021

Assurez-vous que votre profil candidat soit correctement renseigné avant de postuler. Les informations de votre profil complètent celles associées à chaque candidature. Afin d’augmenter votre visibilité sur notre Portail Emploi et ainsi permettre aux recruteurs de consulter votre profil candidat, vous avez la possibilité de déposer votre CV dans notre CVThèque en un clic !

Faites connaître cette offre !

General information

Reference : FR550-BRUMIR-002
Workplace : PARIS 05
Date of publication : Friday, September 17, 2021
Type of Contract : FTC Scientist
Contract Period : 24 months
Expected date of employment : 1 January 2022
Proportion of work : Full time
Remuneration : Gross salary will vary with candidate's experience starting at 3106€/month before taxes (around 2500€ after taxes) for candidate with less than 2 years post-doctoral experience. See the official CNRS salary table for researcher at http://labexdynamo.ibpc.fr/fp-dynamo-paris/
Desired level of education : PhD
Experience required : Indifferent

Missions

Attention: You must not have resided or carried out your main activity (e.g. work or studies) in France for more than 12 months in the 3 years prior to the recruitment date.
You have research experience in the field relevant to the call.

Regulation of bacterial gene expression plays a key role in the rapid adaptation of these microorganisms to ever-changing environments. Work carried out in the last fifty years has for instance highlighted the amazing diversity of translational regulators, e.g. proteins or RNAs, and of the molecular mechanisms of regulation.
The person to be recruited will study the translational control of gene expression in the model bacterium Escherichia coli, focusing on the mRNA elements involved in this process. This analysis will be performed on a few selected genes based on previous results from the host lab, but also at the transcriptomic scale using high-throughput approaches.
PI: Maude Guillier, UMR8261; guillier@ibpc.fr

Related publications :
Jagodnik J., Chiaruttini C. and Guillier M., 2017, Stem-loop structures within mRNA coding sequences activate translation initiation and mediate control by small regulatory RNA, Molecular Cell, 68(1): 158-170.

Activities

- Characterization of the 5' end of the transcripts by primer extension and
extension and Northern blot experiments
- Gene expression studies using fusions with the lacZ or mScarlet gene
- Preparation of ribosomes
- RNA-RNA and RNA-protein interaction studies

Skills

Skills/knowledge :
- Theoretical knowledge of post-transcriptional regulation in bacteria
- General knowledge in molecular biology (cloning, E.coli transformation, etc)
- General knowledge of scientific English

Know-how:
- working with RNA and proteins
- preparation of ribosomes
- high throughput transcriptomic analysis
- classical molecular biology techniques: reporter genes, Northerns, Westerns, etc


The person to be recruited should hold a PhD degree in biology and have more than 3 years experience after the PhD. A solid expertise is expected in bacteriology, molecular biology and RNA biochemistry. Previous experience in ribosome biology and/or bioinformatic analysis of RNAseq data will be considered favorably. Good communication in English is necessary.

Work Context

The person to be recruited will work under the direction of Maude Guillier and will join the group « RNA control of gene expression » within the CNRS unit UMR8261. He/she will interact with all members of the group (4 permanent and 4 non-permanent persons). This team is part of the CNRS unit « Microbial Gene Expression » of the « Institut de Biologie Physico-Chimique », located in the center of Paris.
The COFUND FP-DYNAMO-PARIS programme offers a unique interdisciplinary environment and a fundamental research initiative in the centre of Paris to train the scientists of tomorrow in the field of physico-chemical biology. The goal of the research programme is to integrate knowledge on gene expression, structural and membrane biology, and bioenergetics in bacteria, chloroplasts and mitochondria in the general context of improving our understanding the biogenesis of energy-transducing membranes. These studies will involve cutting-edge technologies including structural biology (NMR, X-ray crystallography and CryoEM), RNA sequencing, mass-spectrometry, synthetic biology, microfluidics, computational modelling and visualization. Post-doctoral fellows will have the opportunity of developing their research projects in a dynamic scientific environment covering three main research themes: i) RNA biology from bacteria to chloroplasts, ii) Membrane system dynamics and iii) computational modelling of molecular assemblies. They will benefit from the supervision of internationally-recognised experts in their fields, a world-wide network of renowned institutions, cutting-edge research infrastructure and state-of-the-art technical know-how that will allow them to hone their scientific skills, make themselves known and increase their competitiveness on the labour market. Specific training will be proposed to ERs through attendance of the EMBO course on “Laboratory Leadership for Postdocs” included in a retreat in Padua, and involvement in dedicated complementary soft skill training events.

Constraints and risks

Fellowship period should start between January 1 and May 31 2022.

Additional Information

The application should be written in English and uploaded at https://emploi.cnrs.fr/. It should include a detailed CV, a minimum of two letters of reference, a letter of motivation describing your short and long term career objectives, and a signed declaration stating that you are in compliance with the mobility rule which stipulates that you have not resided or carried out your main activity (work or studies) in France for more than 12 months during the three years preceding the date of recruitment. In addition, the complete file must also be sent to Bruno MIROUX & Magdalena TORTYNA, respectively director and manager of the project at the following email address DYNAMOcofund@ibpc.fr (Tel: 33 1 58 41 50 64). See details on the website http://labexdynamo.ibpc.fr/fp-dynamo-paris/
The position is intended as an initial step in a research career, and the assessment of the applicants will primarily be based on their research qualifications and their potential as researchers with skills that complement and strengthen on-going research within the partner institutions.
Step 1. The applications of each eligible candidate will be evaluated by independent international experts within the relevant research theme. Eligible applicants will be evaluated based on merits, motivation for the program, academic excellence and personal qualifications such as collaborative and communication skills.
Step 2. A maximum of three candidates will be retained for a video interview with the independent experts for each open position (Nov 2021). After the interview, candidates on the short list will be invited to a conference call with the project supervisor and a DYNAMO board member to define the general scope of the project, the training and the research environment. Candidates will be asked to draft a 3-page project proposal, bringing their own original ideas and will be given 2 weeks to submit it.
Step 3. Each project proposal will be evaluated by one of the independent experts and the DYNAMO board member who evaluated the candidate in the previous steps. The final selection will be made based on the scores obtained at steps 1, 2 and 3. The minimum score must be above 20/40. Top candidate will be offered the postdoc position with a starting date from January 1- May 31, 2022.

The CNRS (Centre National de la Recherche Scientifique) is an equal opportunity employer. Furthermore, FP-DYNAMO-PARIS is committed to promoting the role of women in science and thus explicitly encourages women to apply.

We talk about it on Twitter!