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Reference : FR550-BRUMIR-001
Workplace : PARIS 05
Date of publication : Friday, September 17, 2021
Type of Contract : FTC Scientist
Contract Period : 24 months
Expected date of employment : 1 January 2022
Proportion of work : Full time
Remuneration : Gross salaries will vary with candidate's experience starting at 3106€/month before taxes for candidate with less than 2 years post-doctoral experience.
Desired level of education : PhD
Experience required : Indifferent
Attention: You must not have resided or carried out your main activity (e.g. work or studies) in France for more than 12 months in the 3 years prior to the recruitment date.
You have research experience in the field relevant to the call.
In-depth characterization of the CES regulation coupling Rubisco large subunit synthesis and assembly in Chlamydomonas reinhardtii
PI: Katia Wostrikoff, UMR7141; firstname.lastname@example.org
The host laboratory has a long-lasting expertise in photosynthesis, chloroplast biology and microalgal research and the project aims studying the evolution of energy-producing organelles of bacterial ancestry.
Following endosymbiosis and massive gene transfer from organelles to nucleus, chloroplasts and mitochondria developed regulatory feedback loops coupling the translation of some of their genes to the assembly of their products into the multimeric photosynthetic and respiratory complexes of dual genetic origin. This regulation, coined CES process (for control by epistasy of synthesis), prevents the wasteful synthesis of unassembled subunits, and allows fine-tuning the translation of organelle genes to the assembly of their products. The Rubisco enzyme, playing a major role in CO2 fixation, comprises a chloroplast-encoded large subunit, LS, which is regulated by the CES process.
In plants and green algae, the biogenesis of Rubisco has proven to be quite complex. The synthesis of the chloroplast-encoded LS subunit requires at least one nucleus-encoded organellar trans-acting factor and at least 5 chaperones for its folding, stabilization, oligomerization into a tetramer of dimers and subsequent assembly with SS, to yield the LS8SS8 complex. We recently determined that the LS8 octamer, bound to at least one of the chaperone, acts as a regulator of LS synthesis.
The recruited researcher will further explore the CES process coupling Rubisco chloroplast and nuclear encoded subunits -LS and SS- synthesis and assembly in the green microalga Chlamydomonas reinhardtii.
Wietrzynski, W., Traverso, E., Wollman, F.A., and Wostrikoff, K. (2021). The state of oligomerization of Rubisco controls the rate of synthesis of the Rubisco large subunit in Chlamydomonas reinhardtii. Plant Cell 33, 1706-1727.
Majeran, W., Wostrikoff, K., Wollman, F.A., and Vallon, O. (2019). Role of ClpP in the Biogenesis and Degradation of RuBisCO and ATP Synthase in Chlamydomonas reinhardtii. Plants (Basel, Switzerland) 8.
Wostrikoff, K., Clark, A., Sato, S., Clemente, T., and Stern, D. (2012). Ectopic expression of rubisco subunits in maize mesophyll cells does not overcome barriers to cell type-specific accumulation. Plant physiology 160, 419-432.
Feiz, L., Williams-Carrier, R., Wostrikoff, K., Belcher, S., Barkan, A., and Stern, D.B. (2012). Ribulose-1,5-bis-phosphate carboxylase/oxygenase accumulation factor1 is required for holoenzyme assembly in maize. Plant Cell 24, 3435-3446.
Johnson, X., Wostrikoff, K., Finazzi, G., Kuras, R., Schwarz, C., Bujaldon, S., Nickelsen, J., Stern, D.B., Wollman, F.A., and Vallon, O. (2010). MRL1, a conserved Pentatricopeptide repeat protein, is required for stabilization of rbcL mRNA in Chlamydomonas and Arabidopsis. Plant Cell 22, 234-248.
Wostrikoff, K., and Stern, D. (2007). Rubisco large-subunit translation is autoregulated in response to its assembly state in tobacco chloroplasts. Proc Natl Acad Sci U S A 104, 6466-6471.
-identify the components of this CES regulatory complex, possibly including RNA
-understand the requirement for chaperones in the CES biogenesis pathway
-test whether CES process is a post-endosymbiotic innovation tester si le processus CES.
-General knowledge in biology
-In-depth knowledge of organellar biology
-In-depth knowledge in the fields of biochemistry and protein interactions s
-Knowledge in molecular biology
-Very good knowledge of written and spoken English.
-Basic knowledge in numeric data management
-previous experience in microbiology and/or proteomic will be an asset
-Conceive and realize experimental protocols
-Ability to analyze and restitute information (lab notebook handling, preparation of synthesis tables, preparation of oral presentation).
-Prepare articles to publish in international peer-reviewed journals
Soft and interpersonal skills
-Innovation, thoroughness and reliability in the execution of work.
-Aptitude to work in a team
The work will be led in the host laboratory : UMR7141 “Biologie du Chloroplaste et Perception de la lumière chez les microalgues”, directed by Dr. Angela Falciatore, and the adjunct-director Dr. Yves Choquet, at the IBPC (Institut de Biologie-Physico-chimique) in Paris (http://www.ibpc.fr/UMR7141/en/home/). This laboratory, located in the middle of the Latin Quarter gathers about 30 members, among which 19 with permanent positions. The project will be directed by Dr. Katia Wostrikoff.
The COFUND FP-DYNAMO-PARIS programme offers a unique interdisciplinary environment and a fundamental research initiative in the centre of Paris to train the scientists of tomorrow in the field of physico-chemical biology. The goal of the research programme is to integrate knowledge on gene expression, structural and membrane biology, and bioenergetics in bacteria, chloroplasts and mitochondria in the general context of improving our understanding the biogenesis of energy-transducing membranes. These studies will involve cutting-edge technologies including structural biology (NMR, X-ray crystallography and CryoEM), RNA sequencing, mass-spectrometry, synthetic biology, microfluidics, computational modelling and visualization. Post-doctoral fellows will have the opportunity of developing their research projects in a dynamic scientific environment covering three main research themes: i) RNA biology from bacteria to chloroplasts, ii) Membrane system dynamics and iii) computational modelling of molecular assemblies. They will benefit from the supervision of internationally-recognised experts in their fields, a world-wide network of renowned institutions, cutting-edge research infrastructure and state-of-the-art technical know-how that will allow them to hone their scientific skills, make themselves known and increase their competitiveness on the labour market. Specific training will be proposed to ERs through attendance of the EMBO course on “Laboratory Leadership for Postdocs” included in a retreat in Padua, and involvement in dedicated complementary soft skill training events.
Constraints and risks
The contract must start between 1 January 2022 and 31 May.
The application should be written in English and uploaded at https://emploi.cnrs.fr/. It should include a detailed CV, a minimum of two letters of reference, a letter of motivation describing your short and long term career objectives, and a signed declaration stating that you are in compliance with the mobility rule which stipulates that you have not resided or carried out your main activity (work or studies) in France for more than 12 months during the three years preceding the date of recruitment. In addition, the complete file must also be sent to Bruno MIROUX & Magdalena TORTYNA, respectively director and manager of the project at the following email address DYNAMOcofund@ibpc.fr (Tel: 33 1 58 41 50 64). See details on the website http://labexdynamo.ibpc.fr/fp-dynamo-paris/
The position is intended as an initial step in a research career, and the assessment of the applicants will primarily be based on their research qualifications and their potential as researchers with skills that complement and strengthen on-going research within the partner institutions.
Step 1. The applications of each eligible candidate will be evaluated by independent international experts within the relevant research theme. Eligible applicants will be evaluated based on merits, motivation for the program, academic excellence and personal qualifications such as collaborative and communication skills.
Step 2. A maximum of three candidates will be retained for a video interview with the independent experts for each open position (Nov 2021). After the interview, candidates on the short list will be invited to a conference call with the project supervisor and a DYNAMO board member to define the general scope of the project, the training and the research environment. Candidates will be asked to draft a 3-page project proposal, bringing their own original ideas and will be given 2 weeks to submit it.
Step 3. Each project proposal will be evaluated by one of the independent experts and the DYNAMO board member who evaluated the candidate in the previous steps. The final selection will be made based on the scores obtained at steps 1, 2 and 3. The minimum score must be above 20/40. Top candidate will be offered the postdoc position with a starting date from January 1- May 31, 2022.
The CNRS (Centre National de la Recherche Scientifique) is an equal opportunity employer. Furthermore, FP-DYNAMO-PARIS is committed to promoting the role of women in science and thus explicitly encourages women to apply.
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