Description of the thesis topic

Advancing neural interfaces is of paramount importance across various fields, from deepening our understanding of neuronal function in neurodegenerative diseases to driving the development of pharmaceuticals, and brain–computer interactions. At the heart of this progress of the encoding and decoding of neural activities is the critical need to develop technologies capable of seamlessly integrating neural activity with artificial components. Neurons-on-a-Chip technology has emerged as a valuable toolset, combining two key enabling technologies: PDMS-based microfluidic devices and microelectrode array technology. Microfluidic channel devices through a simple replica molding process within biological laboratories has significantly shortened the turnaround time from assay design to chip fabrication, facilitating various experimental designs. Meanwhile, microelectrode chip technology, which measures electrophysiological signals from individual neurons, has become a popular platform for investigating neural information processing in networks. These advancements have not only enabled the exploration of the intricate relationship between network structure and function but have also ushered in a new era of neurobiology, poised to become standard tools in the near future. However, a current limitation is the weak interaction of planar microelectrodes with neuronal cells which limits the recorded signal quality. At LAAS, we are developing a new class of bio-platform that integrates 3D nanostructured probes (I. Muguet et al 2023 Adv. Mat. 2302472), enabling a very high affinity with interfaced cells while being minimally intrusive due to their low dimensionality. Through meticulous engineering of the probes at the nanoscale, we have achieved very high electrical signal recording (L. Bettamin et al 2024 Small 2309055). In the framework of a newly granted project by the French National Research Agency (ANR) in collaboration with IMS laboratory in Bordeaux, LAAS-CNRS aims to combine these nano-electrodes with microfluidic stamps to design advanced neurons-on-a-chip technology for bidirectional cellular-level interfaces.
Thesis subject: We are looking for a talented PhD candidate who will work on the technological development of nanoelectrode array technology for advanced bio-directional communication with neuronal network at cellular level.
The activities of this PhD project include
(i) The development of the nanoelectrode array platform specifically designed for the bi-directional interfacing of different neuronal networks (Nanotechnology clean room)
(ii) the integration of the neurons-on-a-chip technology including primary neuronal cell culture.
(iii) the relevant neuronal activity recording and the manipulation of activity/connectivity through localized stimulation.

Work Context

With a large number of permanent researchers, engineers and PhD students as well as strong interactions with industry, the LAAS-CNRS is situated at the crossing between scientific research, innovation and applications, where science meets nanotechnology in close connection with society. The LAAS-CNRS possesses one of the most important technological installations dedicated to research in Europe, with an ultra-modern cleanroom of 1600m². and a new biology laboratory for cell cultures, and bio-related activities.
The PhD student will be associated to the nano&neuro electronics activities (within MPN team) that have an international recognition in the development of functional nano-devices for nano- and bio-electronics applications. The project will mainly be carried out in the host laboratory in Toulouse, but the student researcher will work in a very stimulating context of international research collaboration.

The position is located in a sector under the protection of scientific and technical potential (PPST), and therefore requires, in accordance with the regulations, that your arrival is authorized by the competent authority of the MESR.

Constraints and risks

We are looking for a talented and enthusiastic young researcher who will be involved in this project, curious, with a certain autonomy and a strong motivation to develop skills at the interface between nanotechnologies and neuroscience. In addition, the candidate must be able to work in a team on multidisciplinary projects and international environment. Holder of an engineering degree and / or a master's degree, the position requires solid knowledge in nanotechnology and electronic devices and / or neuroscience. Expertise in neuronal cell cultures or electrophysiology would be an advantage. Comprehensive training in other aspects will be provided in the project's multidisciplinary environment. Very good oral and written communication skills in English are required for interactions with project partners but also for presenting at conferences and writing articles in scientific journals.

Additional Information

Applications should include a detailed CV; at least two references (people likely to be contacted); a cover letter of one page; a one-page summary of the master thesis; the marks of Master 1 or 2 or engineering school).