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Reference : UPR2357-SANNOI-002
Workplace : STRASBOURG
Date of publication : Thursday, June 25, 2020
Scientific Responsible name : Sandra NOIR
Type of Contract : PhD Student contract / Thesis offer
Contract Period : 36 months
Start date of the thesis : 1 September 2020
Proportion of work : Full time
Remuneration : 2 135,00 € gross monthly
Description of the thesis topic
The research project focuses on the functions of the F-box FBL17 protein in the model plant Arabidopsis thaliana. The host team previously showed that the F-box protein, FBL17 part of the ubiquitin E3 ligase complex SCFFBL17 is a key regulator of cell cycle progression during both male gametogenesis (Gusti et al 2009) and sporophytic diploid phase of the plant (Noir et al 2015). This F-box protein appears to be involved not only in the regulation of DNA replication, but also in the mitosis control. Additional data have also revealed a change in expression of key genes involved in DNA damage response (DDR), as well as the occurrence of a constitutive DDR in the fbl17 mutant (Noir et al 2015; Gentric et al 2020).
The objectives of the PhD project are to identify protein complexes associated with FBL17 (upon standard and stress conditions), and to analyze post-translational modifications of FBL17 and its putative interactors.
Using the Arabidopsis plant model, the candidate will design and set technical approach to purify these protein complexes. The different levels of analyses to be considered and/or developed will be:
-Cloning, selection and transformation of plants
-Extraction and purification of protein complexes
-Expression of proteins in bacteria
-Analysis of protein-protein interactions (Y2H, GST-pull-down, FLIM)
-Analysis of post-translational modifications (phosphorylation, ubiquitination)
-Analysis of the subcellular localization of fluorescent proteins
The host institute is located in Strasbourg, France. The host team is composed of three CNRS researchers, a research engineer, two Professor assistants, a post-doc researcher and two PhD students. The candidate will work within the framework of the ANR-PRCI RHiD project aiming, in particular, to determine the interactome and/or substrates of the FBL17 protein and the post-translational modifications of these interactors.
The candidate will benefit from the team member's expertise and will join the weekly lab meetings and presentation of the results (mainly in English) and will also attend the institute seminars.
The candidate will have a Master degree in biological sciences, and will present the following skills:
- strong skills in molecular and cellular biology
- experience in plant biology
- expertise in protein biochemistry will be a plus.
- strong motivation, seriousness and assiduity
- fluency in English read, written and spoken
- ability to work independently as well as in interaction with the different team members.
To apply, a motivation letter, a CV (with the contact details of at least one referee) as well as university marks (M1+M2/equivalent) will be requested.
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