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Novel approaches for sensitive detection of doping substances in athletes

This offer is available in the following languages:
Français - Anglais

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General information

Reference : UMR8612-MYRTAV-001
Workplace : CHATENAY MALABRY
Date of publication : Tuesday, July 23, 2019
Scientific Responsible name : Myriam Taverna, Duc Mai et Thuy Tran
Type of Contract : PhD Student contract / Thesis offer
Contract Period : 36 months
Start date of the thesis : 4 November 2019
Proportion of work : Full time
Remuneration : 2 135,00 € gross monthly

Description of the thesis topic

Currently, the screening of a large number of doping substances (DS) in urine of athletes is carried out after non-selective dilution of the sample. This approach is mainly used to screen compounds with limited metabolism and present at relatively high concentration in urine (10-100 ng/mL). In the case of traces of DS, immuno-purification or solid phase extraction (SPE) methods are required, but they are limited due to the possible loss of sample and the risk of cross-contamination. We have recently developed several electrokinetic preconcentration approaches that can be performed by capillary electrophoresis. These preconcentration strategies developed in our group allowed the quantification of biological molecules at extremely low concentrations (Mai TD 2016, C. Crosnier 2018) and open up possibilities for infinite preconcentration of analytes.
Thus, the PhD will develop methods based on these approaches to quantify metabolites or illicit hormonal peptides in urine. To further improve detection limits, these approaches can be combined online with solid phase extraction, using monolith for the enrichment of molecules (Araya-Farias 2017). In this project, we willl use these new approaches to preconcentrate small growth hormone secreatogogue peptides and other molecules found in urine that will be chosen in agreement with the « Agence Française de Lutte contre le Dopage ». The PhD will establish collaborations with this agency to enrich his/her thesis topic and meet the current challenges in anti-doping purposes. Finally, in addition to enrichment and preconcentration methods, the PhD will also develop separation methods based on UHPLC or capillary electrophoresis, possibly coupled to mass spectrometry, for the detection and quantification of these molecules.

References:
Araya-Farias at al., Analyst, 2017, 142, 485, DOI : 10.1039 / c6an02324j
Crosnier et al., Anal Chem 2018, 90, 2555, doi : 10.1021/acs.analchem.7b03843
Mai et al., J Chromatogr A 2016, 1453, 116, DOI : 10.1016/j.chroma.2016.05.048

Work Context

This work will be carried out in one team of the Institut Galien Paris Sud, entitled "Proteins and Nanotechnologies in Analytical Science", specialized in analytical chemistry of biomarkers.

The research unit is composed of 7 common services offering a wide range of advanced analytical and characterization techniques (isothermal calorimetric titration, surface plasma resonance, HPLC...).
http://www.umr-cnrs8612.u-psud.fr/servicescommuns.php

The team has many analytical equipments such as capillary electrophoresis, UHPLC, nanoLC, mass spectrometry, photo- and fluori-meters.
The PhD will be supervised by two persons: Myriam Taverna (Professor in Pharmaceutical Biotechnology) and Thuy Tran (Associate Professor in Analytical Chemistry).
http://www.umr-cnrs8612.u-psud.fr/pres_eq4.php

Additional Information

Required profile:
This topic is intended for students who are motivated by analytical science, particularly separation techniques and mass spectrometry, but also the field of bioanalysis.
The candidate must hold an engineering degree and/or a master's degree. The candidate must have good theoretical knowledge (at least) in analytical chemistry, including separation techniques. An experience in capillary electrophoresis, particularly mass spectrometry, would be an asset.
The candidate must have both oral and written English proficiency. We are looking for motivated candidates who have excellent communication skills, teamworking ability, critical and open mind.

Documents required for application:
Applications must include a detailed CV; at least two references (persons likely to be contacted); a letter of motivation; Master 1 or 2 or engineering mark and possibly a letter of recommendation from a previous training supervisor

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