Reference : UMR8104-FLONIE0-003
Workplace : PARIS 14
Date of publication : Monday, June 20, 2022
Scientific Responsible name : Florence Niedergang
Type of Contract : PhD Student contract / Thesis offer
Contract Period : 36 months
Start date of the thesis : 1 September 2022
Proportion of work : Full time
Remuneration : 2 135,00 € gross monthly
Description of the thesis topic
Phagocytosis is a mechanism of internalization of objects larger than 0.5 microns. How multiple receptors simultaneously recognize microbes, leading to a complex interplay between the signaling pathways and a fine-tuning of the fate of the internalised material, is still not well understood.
This proposal aims at untangling critical steps of phagocytosis in macrophages taking advantage of a multi-disciplinary approach and unique deformable emulsion droplets coated with novel fluorescent receptor-targeted ligands to detect by FRET receptors binding and clustering, and to monitor directly the forces generated by the phagocyte.
The project will focus on receptors for important pathogenic microbes, which have been overlooked so far, mannose receptors, and their cross talk with complement receptors. Important regulators of force generation and phagosome formation will then be identified in primary macrophages using these unique read-outs. This work will thus shed unprecedented and quantitative light on the complexity in phagocytosis mediated by multiple receptors of pathophysiological importance.
The project is supported by the 80 I PRIME (Projet de recherche inter-instituts multi-équipes) CNRS programme (http://www.cnrs.fr/mi).
The PhD candidate will be co-supervised by Florence Niedergang (Team « Biology of Phagocytes, Infection and Immunity» at Institut Cochin, www.institutcochin.fr, INSB) and Jacques Fattaccioli (Institut Pierre Gilles de Gennes – CNRS UMR 8640- INC, ENS Chemistry Department, Sorbonne Université), in close collaboration with Jean-Maurice MALLET (Laboratoire des Biomolécules, CNRS UMR7203- INC ENS, Sorbonne Université).
The principal working place will be Institut Cochin, which is one of the biggest biomedical French Research Center located in the center of Paris. The other laboratories are located in Institut Pierre-Gilles de Gennes, a leading institute dedicated to microfluidics and its applications, and the Chemistry department of ENS/PSL, a top tier French university and research center.
The 3 laboratories are located in the Latin Quarter in Paris, within 15 min walking distance.
Constraints and risks
Have good communication skills to be part of a collaborative multidisciplinary project
Work in BSL2 conditions to prepare primary human cells.
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