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Reference : UMR7264-ISATHE0-002
Workplace : NICE
Date of publication : Wednesday, January 06, 2021
Scientific Responsible name : Stephane Azoulay (CNRS, Lead) Matthew Collins (UCPH)
Type of Contract : PhD Student contract / Thesis offer
Contract Period : 36 months
Start date of the thesis : 1 September 2021
Proportion of work : Full time
Remuneration : 2 135,00 € gross monthly
Description of the thesis topic
The identification of proteinaceous material associated with archaeological ceramics offers much greater resolution in determining vessel contents than lipid based approaches. Their routine analysis would provide an essential tool for understanding the development of early agricultural economies as pioneering research has shown. However, the preservation of proteins as a result of different types of processing (particularly the degree of heating) and different burial conditions (acid, alkali, anoxic) is still unclear. You will receive training in immunological and proteomic analysis, and apply these approaches to experimental, ethno-archaeological and prehistoric ceramics. The student will conduct a series of experiments aimed to investigate different cooking processes and at simulating degradation using the facilities in CEPAM and ICN, Nice and by secondment to UoY to work at the YEAR Centre.
The samples produced will be analysed using a combination of state of the art immunoassay techniques (Luminex® xMAP® technology by Azoulay at CNRS) and proteomic-based approaches (LC-MS/MS at UCPH by Collins), in order to compare results between both techniques. Informed by these experiments, a selection of prehistoric ceramics that have already been analysed for lipids will be made via collaboration with ESR7 and ES9. You will have an opportunity to take a secondment to the University of York to work under the direction of Hendy performing replica cooking and degradation simulations at the YEAR Centre.
The PhD fit within the programm ChemArch https://sites.google.com/palaeome.org/chemarch/home
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