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Reference : UMR5297-MELDES0-016
Workplace : BORDEAUX
Date of publication : Monday, September 09, 2019
Scientific Responsible name : Jean-Baptiste SIBARITA
Type of Contract : PhD Student contract / Thesis offer
Contract Period : 36 months
Start date of the thesis : 1 November 2019
Proportion of work : Full time
Remuneration : 2 135,00 € gross monthly
Description of the thesis topic
The project aims at developing a screening platform comprising: i- a cutting-edge approach of 3D cell culture from pluripotent stem cells (iPSCs), combined with ii- a unique engineering technique for cellular microenvironment control and iii- an innovative soSPIM-based imaging technique allowing long term live 3D imaging of organoids coupled to morphogenetic image analysis.
Activities: Implement a screening platform dedicated to live 3D cell culture based on the soSPIM technology developed in the host laboratory (automated acquisition ans analysis workflow). Handle 3D cell culture models growth in dedicated microfabricated microwells.
Established in January 2011, the Interdisciplinary Institute for Neuroscience (IINS) parent institutions are the CNRS (French National Center for Scientific Research) and the University of Bordeaux.
With 160 people and 12 research teams, IINS deploys multidisciplinary expertise to study molecular and cellular mechanisms involved in brain activity. The Institute develops innovative methods in imaging, chemistry, physiology and computer science. IINS is a major player in Aquitaine's neuroscience community and a leading component of Bordeaux Neurocampus and BRAIN, a cluster of excellence in Neuroscience.
The project will be mainly developed in the host laboratory in Bordeaux, but the PhD student will be exposed to international research. He will have to travel to Singapore to learn 3D cell culture and duplicate the screening platform.
The PhD student will be co-supervised by J.B. Sibarita in Bordeaux and V. Viasnoff in Singapore (MBI, UMI3639 CNRS). Both teams have a long standing and fruitful collaboration (7 years, 3 publications and 4 patents).
Constraints and risks
Work in moved schedule possible.
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