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Reference : UMR3571-PIECOR-005
Workplace : PARIS 15
Date of publication : Tuesday, April 20, 2021
Scientific Responsible name : Pierre-Jean Corringer
Type of Contract : PhD Student contract / Thesis offer
Contract Period : 36 months
Start date of the thesis : 1 October 2021
Proportion of work : Full time
Remuneration : 2 135,00 € gross monthly
Description of the thesis topic
PhD position on “the investigation of pentameric ligand-gated ion channels interaction with lipids and allosteric modulators”. The Channel-receptors group of the Institut Pasteur in Paris downtown is seeking highly motivated candidates for a PhD position in biochemistry to perform structure-function analysis of lipid-membrane protein interaction.
The successful candidate will be recruited for 36 months funded by an ERC doctoral fellowship. The work will involve a large panel of techniques, from the production of pentameric channels in bacteria and in cell lines, their purification, and the study of their interaction with lipids by equilibrium binding experiments, thermic denaturation, ion fluxes and fluorescence. The collected data will allow establishing the “lipid pharmacology” of these receptors, its cross-talk with synthetic allosteric modulators, and will pave the way for structural experiments using the facility of cryo-electron microscopy of the Institut Pasteur.Applicants are expected to have a master in biology, biophysics, biochemistry or pharmacology. Previous experience in biochemistry, especially concerning integral membrane proteins, will be a clear asset. The candidate will perform the work in a multidisciplinary environment, in particular in collaboration with computer scientists and physiologists.
The work will be performed in the “Channel-receptors” team, under the supervision of Pierre-Jean Corringer. The team belong to the CNRS unit “genes synapses and cognition” and to the Neuroscience Department of the Institut Pasteur. The Institut Pasteur of Paris is internationally recognized and offers a highly dynamic social and scientific environment in the heart of Paris. Our lab studies the structure and function of pentameric ligand-gated ion channels, with a particular emphasis on understanding the structural bases of ion channel gating and regulation by small molecules. All projects in the lab are interdisciplinary in nature and combine structural techniques with functional approaches, including biochemistry, crystallography, pharmacology and electrophysiology, for which the lab is fully equipped. For more information on the Channel Receptor group, see (https://research.pasteur.fr/en/team/channel-receptors/)
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