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Reference : UMR2594-ALIBOU-001
Workplace : CASTANET TOLOSAN
Date of publication : Monday, January 11, 2021
Scientific Responsible name : Alice Boulanger
Type of Contract : PhD Student contract / Thesis offer
Contract Period : 36 months
Start date of the thesis : 1 April 2021
Proportion of work : Full time
Remuneration : 2 135,00 € gross monthly
Description of the thesis topic
Xcc (Xanthomonas campestris pv. campestris) is the causal agent of black rot disease on Brassicaceae (Arabidopsis, cabbage …). A recent study performed in our group (Infectious Strategies of Xanthomonas, LIPM) identified genes important for Xcc adaptation during plant infection using a transposon sequencing (TnSeq) screen. The PhD project will include bacterial fitness analyses using RB-TnSeq, transcriptomic analyses by RNAseq and functional characterization of genes in several plant compartments (leaf surface, hydathodes, mesophyll,…) and ex-planta conditions (rich and minimal media, xylem sap). This work will capture a snapshot of the physiology of Xcc during plant infection and lead to (i) characterization of genes functionally important for bacterial fitness in planta; (ii) characterization of transcriptional regulators important for Xcc transcriptional reprogramming throughout the infectious cycle; (iii) identification of pathways important for Xcc adaptation to distinct plant environments.
The Laboratory of Plants-Microbes-environment Interactions (LIPM), UMR2594/441 is part of the National Research Institute for Agriculture, Food and Environment. The lab is located in Toulouse area in the south-west of France.
More information about the team : www.xantho.fr
We are looking for a highly motivated and talented candidate with a background in microbiology, molecular biology and genetics, plant biology and statistics using R. The candidate should hold a Master 2 degree or equivalent.
The ANR funding covers a 3-years contract starting middle of 2021.
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