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Reference : UPR9002-FRAMAR-001
Workplace : STRASBOURG
Date of publication : Friday, February 01, 2019
Type of Contract : FTC Scientist
Contract Period : 7 months
Expected date of employment : 1 March 2019
Proportion of work : Full time
Remuneration : starting at 2555,39 euros per month according to experience
Desired level of education : PhD
Experience required : 5 to 10 years
The team 'Evolution of translation initiation systems in eukaryotes' of the 'Architecture et Réactivité de l'ARN' unit (UPR 9002 CNRS) of the Institute of Molecular and Cell Biology of Strasbourg, is recruiting a Post-doctoral researcher for the study of structure-function relationships of ribonucleic complexes during the translation initiation step in higher eukaryotes. The project will consist in characterizing structurally and functionally macromolecular complexes allowing the initiation of the translation of model messenger RNAs.
The approaches used will be based on the use of cell-free translation extracts that will allow to first assemble and then purify translation initiation complexes on our model messenger RNAs. The composition of these complexes will be determined by mass spectrometry and their structure by advanced structural approaches. The techniques used will be:
- biochemistry and molecular biology methods dedicated to the study of proteins and nucleic acids
- preparation of cell-free extracts for in vitro translation, purification of ribonucleic complexes suitable for structural studies by crystallography / cryo-electron microscopy
- crystallography and / or cryo-electron microscopy
- analysis of electronic density maps
- modeling the atomic structures of proteins / nucleic acids within complexes
The candidate will have to develop his project autonomously and will participate in the supervision of students and technical staff.
Strong expertise in structural biology (Crystallography / Cryo-Electron Microscopy) on macromolecular complexes containing the ribosome is required. This includes mastering biochemistry and molecular biology approaches for complex purification. The candidate should also be able to interpret electronic density maps to establish atomic models of ribonucleic complexes containing the ribosome. Applicants who have demonstrated their ability to write grant applications will be given priority.
The candidate will integrate a team of biochemistry and molecular biology. The implementation of the structural part of this project will be based mainly on the skills of the candidate who will carry out this project independently.
Constraints and risks
The project will require the use of radioactivity and possibly the use of X-rays. Therefore, the mastery of good laboratory practices will be considered as a plus.
Applications will only be made via the recruitment website
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