Reference : UPR9002-ALALES-003
Workplace : STRASBOURG
Date of publication : Monday, August 1, 2022
Type of Contract : FTC Technical / Administrative
Contract Period : 12 months
Expected date of employment : 1 October 2022
Proportion of work : Full time
Remuneration : 2130 euros brut per month
Desired level of education : 5-year university degree
Experience required : Indifferent
The Study Engineer candidate will participate in a research project that aims at characterizing the molecular activity of the selenium-containing protein SelenoN and will be in charge of designing and conducting molecular and cellular biology experiments.
This project is organized in three work-packages: The first one intends to identify bound ligands using biochemical studies of purified recombinant SelenoN protein. In parallel, we will characterize the SelenoN cellular partners using biotin proximal labeling (BioID) or co-immunoprecipitaiton technologies. Based on the information obtained, in vitro enzymatic assays will be designed to test the predicted enzymatic activities. In the third analysis, animal model will be used to integrate the results obtained at a physiological level, in particular in the pancreas. These experiments will be carried out in collaboration with a team of the European Center for Diabetes's Studies in Strasbourg.
The applicants are expected to have a strong background in molecular and cellular biology, including previous experience in recombinant membrane protein expression, chromatographic purification using the Akta system, metabolite analysis using mass spectrometry (LC-MS or GC-MS), invitro culture of eukaryotic cell lines, BioID and co-immunoprecipitation technologies, preparation of isolated pancreas islets and metabolic analysis.
The applicant must hold a certificate for the level I of animal experimentation training.
The ideal candidate should be highly curious and enthusiastic to develop and conduct his/her project independently.
Previously, we have conducted structure-function analyses on SelenoN. Based on the data collected, we are planning to identify the reaction catalyzed by this enzyme and to integrate this activity in its cellular and physiological context.
The candidate will join a biochemistry and molecular biology team entitled 'Post-transcritpionnal Regulation and Nutrition' of the unit 'Architecture and Reactivity of RNA' (UPR 9002 of the CNRS), at the Institute of Molecular and Cellular Biology (IBMC) g. This department is localized on the campus of the University of Strasburg. The lab provides state-of-the-art facilities to perform cellular and molecular biology, biochemistry, as well as Biosafety Level 2 laboratory and animal facility.
Constraints and risks
A large part of the experimental work will be carried out in a Biosafety Level 2 laboratory. Therefore, mastery of good laboratory practices is a requirement. This project also requires a good practice of animal experimentation.
1 year contract
Starting date October 2022
Applications will only be made via the recruitment website. Candidates should send a curriculum vitae, a cover letter (including short statement of research interests) and a concise summary of previous research works, as well as contact details for 2 referees.
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