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Reference : UPR5301-ISACAL-049
Workplace : GRENOBLE
Date of publication : Monday, October 07, 2019
Type of Contract : FTC Scientist
Contract Period : 12 months
Expected date of employment : 1 December 2019
Proportion of work : Full time
Remuneration : 2617.05 €
Desired level of education : PhD
Experience required : Indifferent
Development of a new enzymatic approach to protein glycosylation
- Cloning and expression of proteins in heterologous systems (E. coli, ...)
- Protein purification (affinity chromatography,...)
- Protein analysis (electrophoresis, DLS, fluorescence, MS,...)
- Characterization of enzymatic activities
The candidate must have held a PhD in biochemistry or chemistry for less than 2 years. Skills in glycochemistry or glycobiology will be an asset.
Most proteins undergo post-translational modifications to reach their final destination and assume biological functions. Protein glycosylation is one of the major changes that affect more than 50% of proteins. This modification plays a crucial role in their folding, stability and solubility. It also contributes significantly to cell-to-cell communication. The growing demand for therapeutic proteins and industrial biocatalysts is stimulating intense research on the production of glycosylated recombinant proteins. In this context, the CERMAV teams "Chemistry and Biotechnology of Oligosaccharides" and "Molecular and Structural Glycobiology" propose to implement a new in vitro chemo-enzymatic approach for the glycosylation of recombinant proteins. For this purpose, we are looking for a postdoctoral researcher motivated by an interdisciplinary project at the chemistry-biology interface. The candidate's mission will include producing glycosyltransferases, implementing them in vitro to glycosylate target proteins and characterizing the new proteins obtained.
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