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Reference : UPR2357-MANHEI-003
Workplace : STRASBOURG
Date of publication : Monday, October 11, 2021
Type of Contract : FTC Scientist
Contract Period : 36 months
Expected date of employment : 1 February 2022
Proportion of work : Full time
Remuneration : 2690 -3099 € brutto per months dependent on experience
Desired level of education : PhD
Experience required : 1 to 4 years
The candidate will work in the frame of the ANR-funded project GreenTolerance. GreenTolerance aims to uncover the mechanisms of tolerance, a defense strategy that allows infected plants to stay symptom-free. Tolerance prevents disease by optimizing plant fitness despite ongoing viral propagation. However, the determinants of plant tolerance to RNA viruses are poorly understood. Based on the foundation of results that published in Kørner et al., 2018, Nat Plants 4, 157-164, the candidate will determine how tolerance to RNA viruses differs molecularly amongst Arabidopsis thaliana ecotypes and study how virus-derived and endogenous siRNAs and systemic transport influence plant tolerance to RNA viruses. Understanding the molecular and genetic basis of natural tolerance will help breeders to obtain virus-tolerant, disease-free plants, and thus to reduce crop losses.
- phenotyping / profiling plants of different Arabidopsis ecotypes upon viral infection at the level of symptoms, viral RNA and proteins, and viral and host siRNAs
- phenotyping / profiling plants of different Arabidopsis RNA silencing pathway mutants upon viral infection at the level of symptoms, viral RNA and proteins, and viral and host siRNAs
- profiling of the the activity of the viral suppressor of RNA silencing (VSR) in the context of symptoms, profiling of infection in relation to different VSRs nd VSR mutants
- small RNA profiling of infected plants (sRNA seq)
- immunoprecipitation and analysis of VSR-ssociated small RNAs
- Bioinformatic analysis of small RNA profiles
- proximity labeling and characterization of VSR-associated proteins
Secondary tasks :
- Supervision of PhD and Master students
- Collaboration and discussion of research results with the team members as well as with the project partners and their teams
- Communication of research results (seminars, publications, databases, larger public)
- PhD in plant biology and excellent academic record
- Strong background in plant molecular biology and cell biology
- Specific experience in virology, RNA biology, protein biochemistry, and bioinformatics
- Ability to conceive, execute, and complete research projects
- Ability to design experiments and evaluate results independently and creatively
- Ability to observe sanitary measures in working with plant viruses
- Written and verbal communication skill in English (Level C1 or better)
- Competence to collaborate and work in a team
- Competence of scientific supervision of students
- Competence for presenting research results in scientific meetings and congresses
The Institute of Plant Molecular Biology (IBMP; http://www.ibmp.cnrs.fr/?lang=en) is the largest CNRS research institute in plant science in France. It is associated with the University of Strasbourg and the Doctoral School of Life and Health Sciences. It hosts 16 research teams with 135 collaborators, including 92 permanent staff and currently 43 post-doctoral and doctoral students. The institute is recognized for its expertise in plant virology, genomics, genetics, metabolism, biochemistry, cell biology and molecular physiology. It is equipped with high-end technology platforms staffed for plant production, DNA sequencing and digital qPCR, bioinformatics, proteomics, microscopy, protein production and metabolomics.
The candidate will work in the team and under the responsability of Manfred Heinlein, an internationally renowned scientist interested in identifying the molecular mechanisms of viral movement and antiviral defense in plants and in designing new approaches for crop protection.
Constraints and risks
The project will involve work with radioactive probes.
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