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Reference : UPR2357-CHRRIT-002
Workplace : STRASBOURG
Date of publication : Tuesday, November 17, 2020
Type of Contract : FTC Scientist
Contract Period : 24 months
Expected date of employment : 1 February 2021
Proportion of work : Full time
Remuneration : between € 2,675 and € 3,084 gross monthly depending on experience
Desired level of education : PhD
Experience required : Indifferent
Double-stranded RNA (dsRNA) is regarded as a universal hallmark of infection of cellular organisms by viruses that possess an RNA genome. dsRNA also serves as an essential substrate in the host antiviral response via the innate immune system including the RNA silencing pathway in plants. Although a large number of dsRNA binding proteins have already been identified, mainly in animals, there are no systematic and exhaustive studies at present in plants allowing a complete view of these factors and their functions under normal and pathological conditions.
In the frame of a 4 years ANR-funded project involving the teams of Pascal Genschik, Véronique Ziegler-Graff and Christophe Ritzenthaler (IBMP), our consortium aims at unraveling the molecular mechanisms involved in dsRNA sensing by the host machinery during the infection of plants by viruses. To meet these objectives, we propose to take advantage of new tools developed at IBMP [1-3] to capture dsRNA and associated factors based on the B2-derived dsRNA-binding domain from Flock house virus.
The candidate will purify dsRNA-associated complexes from healthy and virus-infected plants and perform the functional characterization of the identified host components. The study will include systemically and phloem restricted viruses to provide a broad view of ribonucleoprotein complexes and dsRNAs (both cellular and viral) differentially regulated during infection.
- The minimum qualification: PhD degree in molecular biology, biochemistry, structural biochemistry or cell biology.
- The ideal candidate for this position should be highly motivated, have excellent verbal and written communication skills, leadership skills, and independent thinking and problem-solving skills.
- Previous experience in RNA biology, virology, biochemistry or plant genetics will be a plus.
- Candidate must have the ability to work in a multidisciplinary team.
- Candidate must have proven track record of publishing peer reviewed papers.
organizational skills, attention to detail and must possess a strong work ethic.
IBMP provides an excellent scientific environment and is fully equipped with state-of-the art equipment needed to perform the projects, including MS, imaging, sequencing, bioinformatic facilities.
Constraints and risks
Risks associated to molecular biology techniques, biochimistry and manipulation of plants.
Cover letters addressed to Christophe Ritzenthaler must be submitted via the recruitment platform.
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