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Portail > Offres > Offre UMR9198-SEBTHO-001 - Post doctorat sur la nutrition en fer des plantes (H/F)

Post doctoral position on iron nutrition in plants (M/F)

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Français - Anglais

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General information

Reference : UMR9198-SEBTHO-001
Workplace : GIF SUR YVETTE
Date of publication : Thursday, January 10, 2019
Type of Contract : FTC Scientist
Contract Period : 24 months
Expected date of employment : 1 March 2019
Proportion of work : Full time
Remuneration : 2605€ to 3615€ monthy gross salary depending on experience
Desired level of education : PhD
Experience required : Indifferent

Missions

Iron (Fe) is an essential micronutrient for plants productivity as well as the quality of their derived products. However, Fe is generally poorly available to plants because it is mainly present in insoluble forms in soils. Recent studies have demonstrated that Fe nutrition is facilitated by the synthesis and secretion in the soil of phenolic compounds belonging to the coumarin subfamily of specialized metabolites (Tsai & Schmidt, 2017; Fourcroy et al., 2014). The biosynthetic pathway of the secreted coumarins has been elucidated but the mechanisms regulating their secretion remain to be investigated. This is the topic of the ANR project MOBIFER “Dynamics of coumarin secretion by plant roots into the soil to improve iron nutrition”. In this context, the candidate will investigate the mechanisms controlling coumarin secretion in the model plant Arabidopsis in the team “Integrated approaches to ion transport” under the supervision of Michele Bianchi and Sébastien Thomine.

Activities

- The postdoctoral fellow will be involved in the study of the intracellular dynamic of PDR9, the transporter mediating coumarin secretion under iron deficiency, using confocal microscopy and microfluidic devices that allow growing roots on the microscope stage under tight control of nutrition conditions (Agorio et al., 2017; Lanquar et al., 2014).
- The candidate will also be involved in the functional characterization of transporters that mediate coumarin storage in the vacuole in the absence of PDR9, using transport assays and molecular genetics.

Skills

The candidate should hold a PhD degree and have experience in fluorescence microscopy and molecular genetics. He/she should be ready to interact with the other members of our international team and with the other laboratories involved in the MOBIFER project.

Work Context

The candidate will work in the team "Integrated approaches to ion transport" (15 people; https://www.i2bc.paris-saclay.fr/spip.php?article673) which is part of the Department of Cell Biology of the Institute for Integrative Biology of the Cell (I2BC; https://www.i2bc.paris-saclay.fr/spip.php?article312&lang=en) on the research campus of Gif-sur-Yvette. Some experiments may take place at the Synchrotron SOLEIL (Gif-sur-Yvette) and at the Laboratory of Hydrodynamics of the Ecole Polytechnique (LadHyX, Palaiseau).

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