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Reference : UMR9198-NICMIR-001
Workplace : GIF SUR YVETTE
Date of publication : Thursday, August 08, 2019
Type of Contract : FTC Scientist
Contract Period : 24 months
Expected date of employment : 1 October 2019
Proportion of work : Full time
Remuneration : from 31 à 43k€ annually depending on experience
Desired level of education : PhD
Experience required : Indifferent
The postdoc will take place in the team directed by Nicolas Mirouze from the Institute for Integrative Biology of the Cell (I2BC) located in Gif sur Yvette, France.
He or she will be in charge of designing original experimental strategies in order to characterize the genetic transformation process allowing the horizontal transfer of genes in Staphylococcus aureus.
- To design experimental strategies and lead experiments in close interaction with other team members
- To compile results in a lab book
- To keep track of the literrature in the field
- To interact with other team members
- To participate to students mentoring within the team
We are seeking a creative and highly motivated candidate with:
- a PhD in Molecular Genetics, Cellular Biology or any other closely related field
- an excelent experimental experience and strong knowledge in Microbiology
Candidates with the following background are highly encouraged to apply:
- genetic manipulation of Staphylococcus aureus
- diverse microscopy techniques (epifluorescence, high resolution microscopy, image analysis and microfluidics)
Able to work autonomously and to propose original solutions or hypotheses, the candidate selected will have to appreciate team work and display strong communication skills.
A very good level of spoken and written English is required.
The I2BC is a new research institute from the Paris-Saclay University, in a very green and wooded CNRS campus located in Gif sur Yvette, a small city from the Chevreuse valley, 45 min away from Paris by train. The I2BC unit hosts 74 research teams for a total of 700 researchers. Projects in the institute cover a large panel of fields from Genomes Biology, Cellular Biology, Microbiology, Virology, Biochemistry and Structural Biology. The team directed by Nicolas Mirouze is part of the Microbiology department and has access to a number of platforms allowing deep sequencing, mass spectrometry or cellular imaging.
The young host team 'Horizontal gene transfer in human pathogenic bacteria' directed by Nicolas Mirouze (https://www.i2bc.paris-saclay.fr/spip.php?article1464) is composed of 3 people, one researcher, one engeneer and one PhD student. The different projects of the team are all designed to study Horizontal Gene Transfer (HGT) in Gram positive model organisms.
During evolution, the ability of bacteria to adapt to evolving environments often resulted from the acquisition of new genes, and therefore new functions, through HGT. HGT, defined as the transmission of genetic material between organisms that are not in a parent-offspring relationship, is an important means by which bacteria ensure genomic plasticity and acquisition of antibiotic resistance. In bacteria, HGT may occur via three main mechanisms: transduction, conjugation and genetic transformation. The latter mechanism ensures the internalization and homologous recombination within the chromosome of high molecular-weight exogenous DNA. Genetic transformation requires that bacterial cells enter a differentiated state called competence that has been studied in a number of different bacteria, particularly Bacillus subtilis and Streptococcus pneumoniae. Interestingly, new bacteria displaying such aptitude are often discovered, and one of the latest is Staphylococcus aureus.
Our different projects take advantage of multidisciplinary approaches combining Genetic of Prokaryotes, Transcriptional profiling, Protein-protein Interactions and high resolution cellular imaging/microfluidics.
The host team will provide to the candidate all the support necessary for a period of at least 24 months. The project has been financed by a 'Young reasearcher' grant from the ANR. The selected candidate will have to focus on the identification of all the actors involved in natural genetic transformation of the important human pathogen, S. aureus. It will be also important to characterize, in vivo, the spatial and temporal organization of all these actors, allowing HGT in S. aureus.
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