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Reference : UMR9018-FRAAND-001
Workplace : VILLEJUIF
Date of publication : Monday, July 12, 2021
Type of Contract : FTC Scientist
Contract Period : 24 months
Expected date of employment : 15 September 2021
Proportion of work : Full time
Remuneration : Between €2,728 and €3,057 gross per month depending on experience
Desired level of education : PhD
Experience required : 1 to 4 years
The Post-doctoral researcher enrolled in this position, will characterize the in vitro and in vivo effects of specific electrical stimulations on human induced neural stem cells (iNSCs, derived from induced Pluripotent SCs, iPSCs) and multipotent stromal cells (MSCs).
The candidate will optimize specific electric pulses to control the cytosolic calcium oscillations with the aim to facilitate iNSCs maturation into a neuronal lineage and guide MSCs towards a neuronal-like phenotype of cells secreting neurotrophic factors (i.e BDNF, GDNF, etc) essential for neuron differentiation and survival. In addition, in vivo these pulses will work as a physical stimuli to improve the survival of cells in the lesion. The candidate will also develop low amplitude direct currents (DC) stimulation to induce neurites growth and guidance, and limit the secondary inflammatory response of spinal cord after the injury.
The candidate will also investigate the biological effects of these different electrical stimulations (transmembrane potential changes, cytosolic calcium spikes, effects on cell cycle, on differentiation and cellular metabolisms,…) in order to develop their therapeutics application, alone or in combination with chemical factors or nucleic acids.
The successful candidate will have to:
- design and carry out the experiments,
- analyze and interpret the results,
- present the data in writing and orally (English),
- participate in the writing of scientific articles in English,
- supervise students and technicians
The ideal candidate for this position is a highly motivated, excellent researcher with a PhD degree in biophysics or stem cell biology or nerve regeneration. The candidate must master various classical biology techniques: animal cell culture, fluorescence microscopy, flow cytometry, immunohistochemistry, PCR, western blots, etc ...
The candidate should enjoy the challenge of novel scientific concepts and have a highly motivated, persistent and results-oriented attitude. We are looking for candidates interested to work in a multidisciplinary research environment, who have excellent communication skills and are self-motivated, critical and trustworthy. The candidate should be able to work well both independently and in an interdisciplinary team.
Good oral and written communication skills in English are essential.
Good organisational and planning skills are necessary.
The Post-Doctoral fellow chosen for this position will take part in the EU-funded FETOPEN project RISEUP. The RISEUP consortium includes 6 organizations from 3 different European countries, of which one is a SME. Each partner will bring its unique complementary knowhow guaranteeing success of the completion of the Project.
Spinal Cord Injury (SCI), a major cause of paralysis, currently has no effective therapies. Every year almost 500.000 people are diagnosed with SCI worldwide. In Europe, the average investment is 198,000 € in health care during the 1st year/ patient. The difficulty on the neuronal restoration after SCI is based on the complex cascade of events that inexorably cause a degenerative chronic stage mainly favored by the non-permissive environment and limited capacity for axonal regrowth. Multifaceted strategies are considered the unique solution for functional restoration by including cell substitution, neuroprotection and axonal growth promotion. RISEUP proposes to attain neuronal functional regeneration after SCI by an unprecedented and unique bio-hybrid-compatible electro-activated and wireless-rechargeable implantable technology. RISEUP introduces high voltage microsecond electric pulses (micropulses) stimulations and low amplitude direct currents on a combination of stem cells (induced neural stem cells and multipotent stromal cells), whose transplantation is facilitated by an innovative scaffold biomaterial. The RISEUP concept is that micropulses, being able to impose and control cytosolic Calcium oscillations, will facilitate cell maturation, survival and neurotrophic factors secretion. Because Calcium signaling is essential for neuronal activity, endogenous neuronal re-connections will also be favored. RISEUP goal, even if ambitious, is concrete due to the multidisciplinary partners' competences, initiating from TRL1 a radically new line of technology (electro-activated, remotely controlled, biocompatible, biodegradable cell-containing implants for the repair of neuronal lesions) establishing its proof-of-principle (TRL3). The long-term vision of RISEUP is the radical change in SCI treatment modality to assure the cure delivery without any machinery connection, dramatically improving patients' quality of life.
The Post-doctoral researcher will be enrolled by the CNRS and will work in the laboratory UMR 9018 METSY (« Metabolic and systemic aspect of oncogenesis for new therapeutic approaches») under the supervision of Dr Lluis MIR and Dr. Franck ANDRE. These researchers have numerous collaborations in France and in Europe, regularly welcoming interns from these other laboratories. The team also has industrial contacts and a view of promoting research results. The applicant will be working in close collaboration with two others METSY members, also involved in the project: a PhD student and a biological engineer. He/she will participate to all the meetings and activities organized by the international consortium.
The METSY laboratory is located in research pavilion 2 within the Gustave Roussy Institute in Villejuif. The unit is made up of four teams and more than 40 researchers, clinicians, technicians, postdoctoral fellows and doctoral students. The laboratory is equipped with state-of-the-art equipments (e.g. fluorescence microscopy, flow cytometry, Seahorse technology, cell culture, etc) and has full access to technological platforms (e.g. imaging, genomics, transcriptomics, animal facility) managed by the Gustave Roussy Institute.
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