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Reference : UMR9002-BERROB-007
Workplace : MONTPELLIER
Date of publication : Tuesday, January 07, 2020
Type of Contract : FTC Technical / Administrative
Contract Period : 24 months
Expected date of employment : 2 March 2020
Proportion of work : Full time
Remuneration : about 2100€ gross per month, depending on previous experience.
Desired level of education : 5-year university degree
Experience required : Indifferent
Engineer for the molecular study of recombination in meiosis.
The engineer will be in charge of molecular biology experiments, with fine-tuning and optimization if necessary. Parallel analyses using cell biology techniques (microscopy) will also be required. The project will be carried out within a team and the work will involve working effectively with other team members. Discussions and interactions will also be necessary with our collaborators in Montpellier and Lyon. This is an original and innovative project that seeks to explore the diversity of living organisms. The objective will be to obtain a set of comprehensive data for publication.
A first step will be to genotype the Prdm9 gene in different individuals from populations of several species, insects and fishes. The protocol will involve DNA extraction, PCR, sequencing and data analysis. This first part is based on standard protocols. On these species, cytological analyses of meiotic cells will be performed. These analyses will be followed by molecular biology experiments on these cells (chromatin immunoprecipitation or ChIP), with the development of protocols for genomic analyses. New protocols will be tested. If necessary, we will generate antibodies. The engineer will thus carry out the production of the proteins needed for immunization. This will provide the engineer with a large panel of molecular biology expertises.
Good basic knowledge of mathematics, physics, chemistry. Very good knowledge of biology and several months of direct experience in molecular biology. Ability to invest in the work with regularity, tenacity and motivation. Ability to work and interact in a team on a daily basis. Ability to exchange, communicate and discuss. Good level of spoken and written English.
B. de Massy's team is developing research projects on meiosis recombination, molecular mechanisms and their regulation, using genetic, molecular and genomic approaches. A particularly interesting question is to examine the evolutionary conservation of the molecular mechanisms involved. At present, most of our knowledge is limited to "model" species used in the laboratory. Our team has published several papers about the role of Prdm9 in mammals. The proposed project is exploratory, original and innovative and will seek to address this issue in two wild species (fish and insect). This project will be supervised by a researcher with the assistance of a team engineer. This project has been validated and funded and will interact with collaborators who are developing complementary approaches.
Constraints and risks
Bench work, office work. No exposure to pathogens.
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