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Reference : UMR8523-MARDOU0-015
Workplace : VILLENEUVE D ASCQ
Date of publication : Monday, November 25, 2019
Type of Contract : FTC Scientist
Contract Period : 12 months
Expected date of employment : 1 January 2020
Proportion of work : Full time
Remuneration : between 2643.22 € euros and 3766.83 € euros gross monthly according to experience (residence allowance included)
Desired level of education : PhD
Experience required : Indifferent
The Candidate will drive a research project at the interface between physics and biology supported by CPER "Photonics for Societ". He (she) will be in charge of the study by fluorescence microscopy of the response of human cancer cells to oxidative stress. He (she) will build on the previous work of the team that have highlighted mechanisms of feedback that allow these cells to adapt in a short time to an oxidative disturbance. He (she) will focus on the links between the dynamics of degradation of intracellular oxidative species and the activation of survival pathways or cell death. For this, he (she) will conduct experiments on an existing device allowing the tracking of individual cells and the temporal control of an external disturbance by fluidics. He / she will be in charge of conducting the experiments, the automatic analysis of the data by existing image analysis tools in the laboratory and the analysis and synthesis of the results.
The activities of the candidate will be:
- To determine the molecular biology / biochemistry techniques necessary for the realization / integration / validation of bio-sensors.
- To conceive the development and to lead in specialist, the methodological realization
- Validate and interpret the results using biochemistry and / or microscopy techniques
- Evaluate and mobilize the resources needed for the experiments
- Manage the technical resources allocated to experimentation activities
- Apply and enforce the health and safety rules of the domain
- Write technical specifications, design and implementation documents associated with the protocols used
- Keep an experience notebook and follow up on achievements
Deep knowledge of optical microscopy techniques (epi-fluorescence, phase contrast, ...)
Mastery of image analysis techniques, processing and analysis of time series
Mastering the basic techniques of cell culture
General knowledge in physics and in particular on the dynamics of systems
Bibliographic watch (French and English) and improvement / implementation of new technologies necessary for the progress of the project
Master the tools for collecting and processing data (laboratory booklet, statistical analysis)
Have a sense of communication and work in teams or interact with other teams in a multidisciplinary field
Have a good organizational capacity
Organize and facilitate work meetings
Good understanding of written English
Manage orders of consumables and reagents necessary for the implementation of experimentation
Writing academic articles
The project will take place within the "Dynamique des Systèmes Complexes" (DySCo) team of the PhLAM laboratory (UMR8523 / CNRS - University of Lille) in the group "Biophysics of the cellular response to stress". He (she) will work in collaboration with other researchers working on the interface between physics and biology (experimenters and theoreticians). His work, although essentially experimental, will have to be confronted with a mathematical modeling of the phenomena observed. He / she will work in collaboration with a design engineer who will be responsible for the design / construction of the fluorescent bio-sensors used in the project. He (she) will therefore work in a multi-disciplinary environment in order to better understand the link mechanisms of adaptation to oxidative disturbance and cell fate (death / survival).
Constraints and risks
Training provided for the various equipment platforms and a safety training dedicated to physical, chemical and biological risks.The job requires the use of EPI and compliance with the rules of hygiene and safety, as well as the settlement of units
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