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Reference : UMR8231-DIAFRE-003
Workplace : PARIS 05
Date of publication : Tuesday, October 08, 2019
Type of Contract : FTC Scientist
Contract Period : 6 months
Expected date of employment : 4 November 2019
Proportion of work : Full time
Remuneration : between 3000 and 3800 € brut according to professional experience
Desired level of education : PhD
Experience required : Indifferent
The position is offered in the frame of the ANR-funded GEWIEPI collaborative project that brings together the complementary expertise of the groups of Andrew Griffiths and Olivier Tenaillon (Infection Antimicrobials Modelling Evolution / Hopital Bichat, INSERM) in droplet-microfluidics and infectious diseases, respectively. The successful candidate will join a highly multidisciplinary team, with experience spanning biology, chemistry and physics and also work in close contact with the QEM team “Quantitative Evolutionary Microbiology”, part of the Infection Antimicrobials Modelling Evolution Laboratory
The aim of the project is to develop ultra high-throughput phenotyping techniques for bacteria using microfluidics technique, combined with DNA barcoding and high throughput sequencing, for measuring transcriptomic signals in colonies clonal in particular. These techniques will be applied to the characterization of adaptive landscapes in E. coli libraries created at the IAME laboratory.
- Molecular barcoding system design for bacterial transcriptomics
- high-throughput clonal colonies growth system design
- Optimize in-situ retro-transcription in microfluidic systems
- Develop bioinformatic tools for demultiplexing
- Apply these techniques to establish the genotype-phenotype relationship in mutant libraries.
We are seeking a highly motivated engineer to work on single-cell droplet-based microfluidics and next generation sequencing. Requirements are a PhD in molecular biology and/or cellular biology and interest in learning how to use microfluidics systems. Flexibility, autonomy, the ability to work in a highly multidisciplinary team and in a highly interactive research consortium as well as good interpersonal skills are essential.
The research activities of the Laboratory of Biochemistry at ESPCI Paris, directed by Prof. Andrew GRIFFITHS, are based around droplet-based microfluidics, a powerful new ultrahigh-throughput system in which reaction volumes can be miniaturized by up to a million-fold compared to conventional assays in microtiter plates. This opens up exciting prospects for the development of extremely innovative systems with many applications in the Life Sciences.
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