Faites connaître cette offre !
Reference : UMR7564-SOFELK-002
Workplace : VILLERS LES NANCY
Date of publication : Friday, June 4, 2021
Type of Contract : FTC Technical / Administrative
Contract Period : 24 months
Expected date of employment : 6 September 2021
Proportion of work : Full time
Remuneration : 2675€ - 3084€ /month
Desired level of education : 5-year university degree
Experience required : 1 to 4 years
Surface contaminations by microbial biofilms represent major concerns in health and economy. Developing surface coating that prevent microbial growth is still an open challenge.
This project aims to develop antimicrobial surfaces with enzyme-based bio-inspired supramolecular complexes. Antibiofilm enzymes will be grafted on molecular scaffold to obtain a broad spectrum and long term surface protection against microbial contamination. The project relies on a multidisciplinary approach combining biophysics, microbiology, biochemistry, surface chemistry and physical chemistry. Scaffolds with receptors will be grafted on substrates to allow further functionalization with purified antibiofilm enzymes. The candidate will study the interaction between enzymes and scaffold surfaces through methodologies based on AFM (imaging, single-molecule force spectroscopy and single-cell force microscopy) and vibrational spectroscopies (Infrared and Raman). Then, he/she will analyse the interaction of enzymatic scaffolds with bacterial surfaces. Finally, the antibiofilm efficiency will be tested against different bacterial species through several microbiology tests.
Surface chemistry, biophysics and microbiology
Experiments in microbiology, surface functionalization, atomic force microscopy
Candidate holding a PhD (0-2 years of experience) with:
♦ Excellent skills in atomic force microscopy, and more generally biophysics techniques
♦ Strong motivation to work on a multidisciplinary project
♦ Skills in microbiology, AFM, surface chemistry and vibrational spectroscopy
♦ Management skills as the candidate will have to work with a technician and to manage experiments, plannings, reports/articles writing, presentations, meetings
♦ Fluent in English (written, spoken)
♦ Ability to work with different scientific communities
The project is supported by an ANR jcjc grant (SCAZYM). The LCPME is an interdisciplinary lab based in Nancy (France). The candidate will work in a team composed of one technician and 2 researchers: S. El-Kirat-Chatel, expert in microbiology and atomic force microscopy and F. Quilès, expert in physical chemistry and vibrational spectroscopies.
We talk about it on Twitter!