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Reference : UMR7564-CHRMER-003
Workplace : VANDOEUVRE LES NANCY
Date of publication : Wednesday, June 24, 2020
Type of Contract : FTC Scientist
Contract Period : 24 months
Expected date of employment : 14 September 2020
Proportion of work : Full time
Remuneration : gross monthly salary between € 2,675.28 and € 3,084.60 depending on experienc
Desired level of education : PhD
Experience required : Indifferent
The successful candidate will take part in the European project ANTIVERSA aiming at understanding the invasion of natural microbial communities (aquatic or from soil) by antimicrobial resistance genes and/or their associated mobile genetic elements, when exposed to extracellular DNA, transducing phages/vesicles or resistant bacteria of anthropogenic origin. The expected work will cover (i) biodiversity studies on natural microbial communities, and (ii) microcosm studies designed to monitor the spread of antibiotic resistance genes in natural microbial communities collected in the environment. These community invasion studies will implement a panel of molecular approaches specially adapted to the tracking of horizontal gene transfer events by different pathways (e.g. transformation, transduction, conjugation). This work will be carried out in collaboration with microbiologists from a European consortium, all involved in the study of antibiotic resistance dissemination in the environment, but with competencies ranging from molecular ecology to microbial genetics through environmental microbiology. The goal of the consortium is to identify key environmental parameters that drive the spread of antimicrobial resistance in natural communities, especially when they are altered and exposed to anthropogenic pressures.
- Collecting and processing of environmental samples (soils, aquatic biofilms)
- DNA extraction from microbial communities
- Biodiversity analyses of microbial communities (metagenomics)
- Maintaining environmental communities in controlled microcosms (bioreactors)
- Microcosm-based invasion experiments after inoculating antibiotic resistance genes as free DNA, environmental phages, or a panel of resistant bacteria
- Quantitative analysis of resistance gene dissemination by molecular biology (e.g. qPCR)
Trained as microbiologist, the successful candidate must show solid technical and theoretical skills in environmental microbiology and molecular biology. A good practice of quantitative PCR is essential. Since horizontal gene transfer is central to the project, it is understood that the recruited person exhibits a solid knowledge in bacterial genetics to understand the mechanisms involved (conjugation, transduction, transformation) in the complexity of natural systems. Some know-how in cloning techniques is also expected since genetic modifications of bacterial strains (inoculum) will be necessary to monitor the invasions of antibiotic resistance genes in natural communities. The successful candidate must present a good experience in environmental microbiology or ecology (at least at the doctoral level), and must be able to converse in French or English.
The successful candidate will join the LCPME (UMR 7564 UL-CNRS), which gather 78 people distributed in three teams. The recruited person will join the Environmental Microbiology team on the research topic "dissemination of antimicrobial resistance in the environment", under the direction of Christophe Merlin and Xavier Bellanger. The project to which the recruited person will be associated to will take place in the context of an international collaboration bringing together 6 partners collectively aiming to shed light on the importance of the microbial biodiversity in the dissemination of antimicrobial resistance genes in the environment. Strong interactions within LCPME, but also between European partners, will be expected for a harmonious progress of the project between the members of the consortium.
Constraints and risks
No particular risk for the person
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