Reference : UMR7283-DELLER-056
Workplace : MARSEILLE 09
Date of publication : Friday, June 17, 2022
Type of Contract : FTC Scientist
Contract Period : 30 months
Expected date of employment : 1 October 2022
Proportion of work : Full time
Remuneration : between 2743,70 and 3896,73€ gross monthly depending on experience
Desired level of education : PhD
Experience required : Indifferent
The post-doctoral fellow will be involved in a collaborative research project (D2OX) that aims to provide a complete picture of the distribution and dynamics of the actors of an electron transport chain in the model bacterium Escherichia coli. He/she will use advanced fluorescence microscopy approaches at the single cell scale under normal and perturbed conditions. He/she will perform genetic constructs to disrupt the system. He/she will interact with a team of theorists to use the measured parameters for the development of mathematical models describing the spatial organization of the actors of respiration and the kinetic impact on the process.
Primary activities of the successful candidate:
- Design and implementation of experiments in the D2OX project;
- Acquisition, analysis and interpretation of experimental data and proposal of relevant alternative solutions when necessary;
- Presentation of progress reports and results at laboratory/consortium meetings and national/international scientific conferences;
- Contribution to the writing of activity reports, scientific publications and grant applications related to the project;
- Regular monitoring of the scientific literature;
- Contribution to the training and supervision of students in the laboratory.
We are looking for a highly motivated and collaborative candidate with a strong background in molecular biology, microbiology and fluorescence microscopy. Experience in image data analysis or computational modeling of recorded signals would also be appreciated but not essential, as training will be provided. We expect a candidate to have a PhD in life sciences (microbiology, genetics or similar), knowledge of biophysics and to have published in high-ranked journals.
- Experience in microbiology and molecular biology
- Knowledge and experience in fluorescence microscopy
- Ability to work in a collaborative and interdisciplinary environment
- Good autonomy and strong organizational skills
This project will be carried out in a transversal environment combining physics and biology between the teams of Axel Magalon (LCB, Marseille https://lcb.cnrs.fr) and Didier Marguet (CIML, Marseille http://www.ciml.univ-mrs.fr/). The two supervisors have a very complementary expertise in bacterial genetics, biochemistry, microbial physiology, advanced microscopy and signal processing, offering a stimulating environment for the candidate. The two laboratories are located in Marseille, 6 kms away from each other.
Compartmentalization and the emergence of physical barriers are fundamental to life and have been crucial steps in the evolutionary process. Despite decades of study, how oxidative phosphorylation (OXPHOS) is spatially and temporally organized in the membrane for efficient energy conservation remains largely unknown. We have recently demonstrated that several membrane-associated OXPHOS complexes are unevenly distributed in the bacterial cell. Our working hypothesis is that the clustering of OXPHOS complexes into nanodomains facilitates quinone turnover and explains the efficient functioning of bacterial OXPHOS. The overall goal of this project is to further our knowledge of the organization of OXPHOS in bacterial cells and to understand at the molecular level the biological importance of the spatio-temporal organization of OXPHOS. To this end, you will develop and use spatially and temporally resolutive high end fluorescence microscopy approaches at the single cell level under perturbed and normal conditions. These measurements will be combined with computational tools and feed theoretical models to delineate how dynamic spatial organization impacts OXPHOS outcomes.
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