Reference : UMR7277-CHRBRA-015
Workplace : NICE
Date of publication : Wednesday, May 4, 2022
Type of Contract : FTC Scientist
Contract Period : 18 months
Expected date of employment : 1 July 2022
Proportion of work : Full time
Remuneration : 2690€ - 3993€
Desired level of education : PhD
Experience required : Indifferent
Preliminary work shows that RNA phase separation in the C. elegans germline displays extensive environmental and evolutionary variability, suggesting that phase separation is of likely evolutionary ecological relevance. In this collaborative project, we therefore propose (1) to test if observed plasticity in the induction, composition and structure of RNA granules is involved in tuning RNA expression in response to ecologically relevant temperature variation; (2) to test if natural genetic variation in C. elegans RNA phase separation and its thermal sensitivity reflects evolutionary variation consistent with adaptation to divergent ecological niches; (3) to genetically map and molecularly characterize natural genetic variation in RNA phase separation taking advantage of existing differences between wild isolates; (4) to test if and how plasticity in RNA phase separation in response to thermal stress improves organismal fitness.
The objective of the proposed project is to focus on natural variation in stress-induced granule formation and to quantify organismal fitness consequences associated with variation in granule formation. The postdoc will apply C. elegans genetics and mutant analysis, high-throughput phenotyping, molecular biology (e.g. single-molecule FISH), advanced microscopy, and bioinformatic approaches.
- Planning and execution of experiments
- Genetic analysis of C. elegans
- Mutagenesis (CRISPR/Cas9)
- Analysis of "omics" data
- Documentation and interpretation of results; presentation at scientific conferences
- Writing of manuscripts for publications
The candidate should have expertise in bioinformatics, molecular biology and C. elegans biology as well as background in multidisciplinary research at the interface of developmental genetics, cell biology and evolutionary biology.
Constraints and risks
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