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Portal > Offres > Offre UMR7275-BRUANT-001 - Ingénieur de recherche H/F en culture de sphéroides, caractérisation d'organelles cellulaires et lipidomique

Research engineer in sphéroid culture, organelle purification and lipidomics

This offer is available in the following languages:
Français - Anglais

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General information

Reference : UMR7275-BRUANT-001
Workplace : VALBONNE
Date of publication : Saturday, February 01, 2020
Type of Contract : FTC Technical / Administrative
Contract Period : 36 months
Expected date of employment : 1 April 2020
Proportion of work : Full time
Remuneration : Monthly raw salary: 2400 to 2600 € depending on the CV/expertise of the candidate
Desired level of education : PhD
Experience required : 1 to 4 years

Missions

To develop robust and comprehensive protocols for the biochemical characterization of adipocyte spheroids and their lipid droplets with the aim to decipher and understand variations between samples from different origins (e.g. different metabolic state, different genetic backgrounds).

Activities

1- Spheroid cultures
2- Cell biology
3- Organelle purification
4- Biochemical analysis - lipids and proteins (in link with the mass spectrometry platform of the Institut de Pharmacologie Moléculaire et Cellulaire, UMR 7275)

Skills

- Molecular and cellular biology, including microscopy
- biochemical characterisation, organelle purification, lipid and protein extraction.
- Lipidomics (sample preparation, High Performance Thin Layer Chromatography, strategy, mass spectrometry, data analysis)
- Proteomics (sample preparation, mass spectrometry, strategy, data analysis)
- Deep survey of the scientific and technical litterature
- excelllent skills in scientific presentation and writing
- deep knowledge in lipid chemistry and diversity in biology
- Coordination, team work

Work Context

The Institute of Molecular and Cellular Pharmacology (UMR 7275 between the CNRS and the University of the Côte d'Azur) conducts fundamental research that can have repercussions in molecular and cellular medicine. The IPMC is located in the technopole of Sophia Antipolis, includes around twenty research teams, different platforms notably in genomics, microscopy and mass spectrometry and includes around 220 people.
The engineer will join Bruno Antonny's team "Dynamics of membranes and protein coats", comprising a dozen people (4 PhD students, a post doc, a lecturer, 4 researchers).
The work is part of an ERC - synergy research program called SPHERES uniting several research teams in France and Sweden. The objective is to understand the mechanisms controlling the size of adipocytes and in particular their lipid droplets using a 3D culture model of spheroid type.

Constraints and risks

Through missions to France and Sweden, the engineer (M / F) will have to regularly base his skills on spheroids and interact with the other members of the synergy program.

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