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Reference : UMR7257-YVEBOU1-001
Workplace : MARSEILLE 09
Date of publication : Tuesday, October 12, 2021
Type of Contract : FTC Scientist
Contract Period : 24 months
Expected date of employment : 1 February 2022
Proportion of work : Full time
Remuneration : 2743 to 3896€ gross monthly salary depending on experience
Desired level of education : PhD
Experience required : Indifferent
Structural biology project aiming at characterizing the structure-function relationships of enzymes involved in the degradation of heparan sulfate (HS) in collaboration with the teams of Kevin Baranger (LIENSs, La Rochelle) and Franck Morel (LITEC, Poitiers). In particular, we aim to understand how the action of oligosaccharides or protein partners can modulate enzymatic activity in order to decipher the molecular mechanisms of certain cutaneous inflammatory diseases.
- Production and purification of targeted proteins
- Biophysical characterization of proteins and protein/protein-ligand complexes
- Determination of 3D structures of proteins and complexes by cryo-EM or X-ray crystallography
He/she should have a thesis degree (or in the process of finalization) associated with publications in a field relevant to the project. Expertise in recombinant protein expression, particularly in eukaryotic systems, protein biochemistry, and single particle cryo-EM or X-ray crystallography is essential.
The Architecture and Function of Biological Macromolecules (AFMB) laboratory, supervised by the CNRS and Aix-Marseille Univ and in partnership with INRAE, is internationally recognized in the field of structural biology. It is located in the Luminy campus in the south of Marseille, where it benefits from an exceptional scientific, technological and educational environment. Research themes at the AFMB laboratory range from glycobiology and glycogenomics to microbiology, virology and neurobiology. The common objective is the detailed description of the structure-function relationships of proteins, protein-ligand complexes and macromolecular assemblies using modern multidisciplinary approaches in bioinformatics, genetic engineering, biology/biochemistry, biophysics, medicinal chemistry and structural biology.
Constraints and risks
He/she is invited to submit i) a cover letter describing research interests, ii) a CV and iii) names and contact details of three referents.
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