Reference : UMR7242-GAEMIS-003
Workplace : ILLKIRCH GRAFFENSTADEN
Date of publication : Monday, August 1, 2022
Type of Contract : FTC Scientist
Contract Period : 18 months
Expected date of employment : 1 November 2022
Proportion of work : Full time
Remuneration : Starting with 2690 euros gross salary per month, depending on the professional experience
Desired level of education : PhD
Experience required : 1 to 4 years
- Synthesis and characterization of functionalized analogues of bacterial metabolites
-Synthesis and characterization of conjugates between analogues of bacterial metabolites and phospholipids.
- Use of phospholipid conjugates for the preparation of echogenic microbubbles as a contrast agent and antibacterial sound therapy (sonobactericide)
- Organic synthesis (main task)
- Purification and spectroscopic characterization (NMR, MS) of the compounds obtained (main task)
- Scientific communication (oral presentations, writing articles) (secondary task)
- Contribution to the technical life of the laboratory alongside other personnel: (purchasing, maintenance of scientific equipments, management of stocks and reaction waste) (secondary task)
- Level : PhD or PhD +1 or 2 years postdoc
- Practical and theoretical knowledge of organic synthesis
- Skills in chemistry/purification of lipid compounds would be an asset
- Practical and theoretical knowledge spectral analysis techniques (NMR, MS, etc.).
- Mastering tools related to chemistry (electronic lab notebook, drawing software, etc.)
- Knowledge, at least theoretical, in biology/microbiology/biochemistry and wave physics would be considered as an asset.
- Ability to work/evolve in a multidisciplinary environment at the interface of chemistry, biology and physics.
- Good knowledge of the English language (read, written, spoken, B2 level min).
The team "Metals and Microorganisms: Biology, Chemistry and Applications" (MMBCA) is member of the UMR7242 Biotechnology and Cell Signaling located at the Ecole Supérieure de Biotechnologie de Strasbourg (ESBS) located at the illkirch innovation center (10 km from the city center of Strasbourg). This team is composed of chemists and biologists (20 people including 9 permanent positions) developing project at the interface of biology and chemistry with a particular focus on iron homeostasis in pathogenic bacteria. In this context, the research axis dedicated to the targeting of lipid supramolecular systems by bacterial metabolites is currently a booming field. The team has two chemistry laboratories. Each researcher has for his own use a hood, a rotary evaporator and a diaphragm pump. Each of the two laboratories is equipped with an automated purification system (Chromatoflash). A HPLC, a cryostat (-40°C) and a microwave reactor are in common access. The analysis service (NMR, SM) is located in a nearby building.
Constraints and risks
Risks inherent to the synthetic organic chemistry. Trips and stays (sup. to two weeks) with our partner at the Erasmus Medical Center (Rotterdam, Netherlands).
Antibiotic resistance will be a major health challenge in the forthcoming decades. The development of new strategies to struggle against infections is therefore an urgent necessity, particularly to eliminate bacterial biofilms infecting implanted medical systems (catheters, implantable chambers, prostheses, heart valves). The use of sound to destroy bacteria and their biofilms (sonobactericide) is still in its infancy and could be an alternative to surgery on fragile patients. Echogenic microbubbles can be used to detect the site of infection by ultrasound (contrast agent) but also to treat the infection. Indeed, under the effect of ultrasound, the microbubbles vibrate and the shear forces generated dislocate bacteria and biofilms present in the neighborhood of microbubbles. This technique has for the moment the disadvantage of being not very specific to bacterial cells vs human cells. In order to increase the therapeutic potential of this approach we aim to produce by organic synthesis conjugates between bacterial metabolites and phospholipids. For this project a postdoctoral researcher position is proposed under the direction of Dr. Gaëtan Mislin (Research Director at CNRS, Hab. University of Strasbourg). Metabolite-phospholipid conjugates will form the envelope of echogenic microbubbles capable of interacting more specifically and for longer with bacteria and biofilm with therefore limited impact on the patient's cells.
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