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Portail > Offres > Offre UMR7238-JUDMIN-001 - Post-doctorat sur les mécanismes de réparation des cassures de l'ADN (H/F)

Post-doc: Mecanisms of DNA repair at the single molecule level

This offer is available in the following languages:
Français - Anglais

Date Limite Candidature : mardi 11 octobre 2022

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General information

Reference : UMR7238-JUDMIN-001
Workplace : PARIS 05
Date of publication : Tuesday, September 20, 2022
Type of Contract : FTC Scientist
Contract Period : 24 months
Expected date of employment : 1 January 2023
Proportion of work : Full time
Remuneration : 2890
Desired level of education : PhD
Experience required : Indifferent

Missions

The post-doctoral fellow will have to carry out a project on the repair mechanisms of DNA double-strand breaks in human cells. The project aims to investigate the specific role of histone ADP-ribosylation in three steps of DNA repair known to be controlled by this signaling pathway: i) modulation of chromatin architecture, ii) recruitment repair factors and iii) controlling the release of PARP1 from damaged DNA. To study these different aspects of the repair process, we use super resolution microscopy approaches (single molecule tracking) in human cells.

Activities

The post-doc will have to carry out the various experimental stages of the project as well as the 1st stages of microscopy image analysis. These activities include:
- human cell culture in the L2 laboratory (maintenance of lines without the construction of new lines),
- performing fluorescence microscopy experiments (classical and single molecule),
- analysis of single molecule tracking data in matlab and python.

Skills

The post-doc should have in-depth knowledge
- in cell biology, in particular on DNA repair mechanisms.
- a good knowledge of fluorescence microscopy is required.
- an strong interest for biophysical approaches in particular concerning liquid phase separation models.

The post-doctoral fellow must master the following techniques:
- Culture of human cells in L2
- fluorescence microscopy experiments (classical and single molecule),
- analysis of single molecule tracking data in matlab and python.
- knowledge of static analysis and biophysical modeling is appreciated.

The doctoral student must demonstrate:
- very good organizaiton skills,
- a very good level in written and oral English,
- the ability to organize and present their results orally in a clear manner.

Work Context

The post-doc will be carried out in a new ATIP team (“Imaging Nuclear Organization and DNA repair” team) within the Computational and Quantitavie Biology (LCQB) laboratory, IBPS, on the Sorbonne University campus. The IBPS has an environment very rich in interactions between biologists, physicists, computer scientists. The project benefits from strong support from the IBPS microscopy platform.

Constraints and risks

The project required to work in L2 and to use a super resolution microscope containing powerful lasers, these not being visible outside the exit to the objective. No particular risk is to be mentioned.

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