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Reference : UMR7210-VALEMI-002
Workplace : PARIS 12
Date of publication : Thursday, November 12, 2020
Type of Contract : FTC Scientist
Contract Period : 24 months
Expected date of employment : 1 January 2021
Proportion of work : Full time
Remuneration : 2800-3300 € bruts/month according to the level of experience
Desired level of education : PhD
Experience required : Indifferent
Responsible of the design, building up and validation of an optical microscope for fast functional imaging and optogenetics photostimulation.
- Design, build up and characterize a two-photon microscope for fast functional imaging and photostimulation comprising multiple optical paths (two and three photon excitation, two photon endoscopy).
- Validation of the system in proof of principle experiments (mice and zebrafish larvae)
We are seeking a skills motivated candidate with a PhD in Physics, with the following skills:
- demonstrated experience in imaging, optical microscopy and wave front shaping -demonstrated experience in presenting data at international conferences and ability to synthesize and write scientific documents.
- good knowledge of the English language
- demonstrated working experience in interdisciplinary environment (biology-physics)
The Vision Institute is an interdisciplinar research centre affiliated to Sorbonne University, and supported by INSERM and CNRS. It is located on the campus of the National Hospital Centre for Ophthalmology of XV-XX (CHNO des XV-XX). It was created in 2008 by Pr Sahel, the current director, with the aim of bridging academic, private and clinical researches. It is organized in 5 departments and 21 research teams
The institute has facilities for cell culture and cultures of purified neurons. It also has molecular biology facility for virus design, purification and production. It is equipped with a stereotaxic injection facility for AAV injection in mice retina and brain and an animal facility.
The Emiliani team disposes of 8 state of the art optical microscopes, including 3 systems for in vitro 2P-holographic photoactivation and electrophysiology, 2 set up for in vivo 2P- holographic/imaging and electrophysiology and 2 holographic endoscopes for 2P imaging and photoactivation in freely moving animals. They have also recently received the funding (from ille de France; DIM ELICIT) to set up a common facility for 2P-optogenetics in collaboration with the company 3i and a new optical microscope for in vivo 2P and 3P imaging and photostimulation (financing: ERC Advanced grant).
The group is also equipped with an electro-mechanical workshop including a 3D printer machine for the fabrication of small microscope elements and has the facility for brain slicing and craniotomy.
Constraints and risks
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