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Reference : UMR7083-GUIGIN-003
Workplace : PARIS 05
Date of publication : Monday, October 4, 2021
Type of Contract : FTC Scientist
Contract Period : 12 months
Expected date of employment : 1 November 2021
Proportion of work : Full time
Remuneration : 32-47 k€ annual gross salary
Desired level of education : PhD
Experience required : Indifferent
Our team is looking for a talented and motivated postdoc to work on microRNA detection technologies. The postdoctoral position, funded by an ERC starting grant (MoP-MiP), is open at the Gulliver laboratory (ESPCI Paris, PSL Research University). Droplet digital nucleic acid detection relies on the compartmentalization of individual targets into water-in-oil droplet prior to target amplification. Despite the multiple advantages (absolute quantification, high sensitivity, calibration-free), such assays rely on complex microfluidic setup that limit sample throughput and accessibility to non-expert users. Templated emulsions have been developed to palliate these limitations. Here, hydrogel particles are used as pre-compartments that can be quickly sealed by mixing them vigorously with oil. Using monodisperse particles allows to create monodisperse, microfluidics-free droplets.
The candidate will develop and optimized this technology and adapt it to the original amplification method our team has developed for microRNA quantification. In addition, the use of fluorescently-barcoded particles will allow the adaptation of the assay to a multiplex readout.
Background information: https://blog.espci.fr/guillaumegines/erc-mop-mip/
Publications or interest : https://blog.espci.fr/guillaumegines/publications/
Literature on templated emulsion :
Abate group : https://pubs.acs.org/doi/10.1021/acs.analchem.8b01759
Di Carlo group: https://www.biorxiv.org/content/10.1101/2020.03.09.984245v1
- Literature review and data compiling
- Planification and realization of biochemical experiments (assembly of biochemical reaction networks)
- Hydrogel particle synthesis using droplet microfluidics
- Data analysis and regular (weekly) reporting
- Scientific communication (writing of scientific publications, conference attendance)
PhD in biochemistry, chemistry, molecular biology or a related field.
- The candidate is expected to have a strong background in standard molecular biology technique (enzymatic assays, DNA amplification, RNA extraction, microscopy…)
- Excellent communication skills in both written and spoken English are of prime importance
- The successful candidate is expected to navigate in a highly interdisciplinary environment and have independent thinking
- A previous experience in the field of DNA computing, molecular programming or microfluidics.
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