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Reference : UMR5536-PHIMEL-001
Workplace : BORDEAUX,BORDEAUX
Date of publication : Friday, September 25, 2020
Type of Contract : FTC Scientist
Contract Period : 24 months
Expected date of employment : 1 December 2020
Proportion of work : Full time
Remuneration : between 2648€ and 3054€ gross monthly according to experience.
Desired level of education : PhD
Experience required : 1 to 4 years
Persistent protease activity in tissues is an early marker for many pathologies (Inflammation, COPD, cystic fibrosis, cancer, acute and chronic pancreatitis…). Starting in 2005 and thanks to two ANR grants we successfully designed contrast agents for in vivo MRI that are responsive to protease activity. However these contrast agents still need to be optimized for a better biodistribution and persistence in the targeted compartments (lungs, blood…). Furthermore the imaging experiment currently done at 0.2 tesla must be transposed to the new earth field MRI prototype currently being assembled as part of the European grant “PRIMOGAIA”.
First the recruited post-doctoral scientist will measure the Michaelis constants of each substrate newly synthetized by our partner for the Institut de Chimie Radicalaire de Marseille with its target protease, each corresponding to a targeted pathology.
He/she will have to optimize the experiments at 0.2 tesla and then transpose these experiments to the new earth field prototype MRI.
-Enzyme activities measurements.
-Use of electron spin resonance spectroscopy since our substrates are stable free radicals.
-Taking part to the design of new substrates using molecular docking.
-Set up and use of pathology models on mice (lungs inflammation, pancreatitis, solid tumors).
-Taking part of the in vivo MRI experiments (contrast agents delivery, anesthesia, placement into the MRI instrument)
-Collaboration with physicists and chemists
-Research experience in biochemistry.
-Research experience in quantitative enzymology is welcome but can be acquired here.
-A minimum understanding of nuclear magnetic resonance and/or electron spin resonance.
-A qualification for animal experiments.
-experience in cell culture could help.
The job is based at Centre de Résonance Magnétique des Système Biologiques (CRMSB, UMR 5536 CNRS/université de Bordeaux). We are a rare multidisciplinary structure organized around Magnetic resonance Imaging. The associated imaging facility runs clinical and pre-clinical MRI. We also have unique experimental devices for Overhauser –enhanced MRI and earth field MRI. At the same time we have a fully equiped biochemistry lab and cell culture rooms. We also run our own animal facility.
The scientist will be part of the “Proteolysis Imaging” team which closely works with two biochemists, the physicists of the Overhauser-enhanced MRI project and those of the earth field MRI project.
Constraints and risks
-no specific risk
-Work with rodents
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